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- Clone
- QA18A42 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood lymphocytes were stained with purified anti-human CD62L recombinant (clone QA18A42) (left) or purified mouse IgG1, κ isotype control (right) followed by goat anti-mouse IgG PE. Cells were co-stained with CD3 APC.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 306902 | 100 µg | £70 | ||||
CD62L is a 74-95 kD single chain type I glycoprotein referred to as L-selectin or LECAM-1. It is expressed on most peripheral blood B cells, subsets of T and NK cells, monocytes, granulocytes, and certain hematopoietic malignant cells. CD62L binds to carbohydrates present on certain glycoforms of CD34, glycam-1, and MAdCAM-1 and with a low affinity to anionic oligosaccharide sequences related to sialylated Lewis X (sLex, CD15s) through its C-type lectin domain. CD62L is important for the homing of naïve lymphocytes to high endothelial venules in peripheral lymph nodes and Peyer's patches. It also plays a role in leukocyte rolling on activated endothelial cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Immunogen
- Concentrated supernatant from PMA-activated human peripheral blood leukocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
QA18A42 is able to completely block the binding of clone DREG-56 on target cells.
- RRID
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AB_2888837 (BioLegend Cat. No. 306902)
Antigen Details
- Structure
- Selectin, single chain glycoprotein, 74-95 kD
- Distribution
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Majority of B cells, naïve T cells, subset of memory T and NK cells, monocytes, granulocytes, thymocytes
- Function
- Leukocyte homing, leukocyte tethering, rolling
- Ligand/Receptor
- CD34, GlyCAM, MAdCAM-1
- Cell Type
- B cells, Granulocytes, Leukocytes, Lymphocytes, Monocytes, Neutrophils, NK cells, NKT cells, T cells
- Biology Area
- Adaptive Immunity, Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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1. Kishimoto T, et al. 1990. P. Natl. Acad. Sci. USA 87:2244.
2. Kishimoto T, et al. 1991. Blood 78:805 - Gene ID
- 6202 View all products for this Gene ID
- UniProt
- View information about CD62L on UniProt.org
Related FAQs
Other Formats
View All CD62L Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD62L Recombinant Antibody | QA18A42 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD62L Recombinant Antibody
Human peripheral blood lymphocytes were stained with purifie...
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