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- Clone
- VIM8 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Ceramide dodecasacharide, VIM2
- Isotype
- Mouse IgM, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Human peripheral blood leukocytes were stained with purified anti-human CD65 (clone VIM8) (left) or purified mouse IgM, κ isotype control (right), followed by anti-mouse IgM PE.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 351402 | 100 µg | £151 | ||||
CD65, also referred to as ceramide-dodecasaccharide or type II fucoganglioside, is a fucosylated carbohydrate antigen which serves as a ligand for CD62E, commonly known as E-selectin. A key difference between CD65 and CD65s is that CD65 does not have a terminal sialic acid, although the rest of their structures are identical. CD65 is expressed on most myeloid cells during development, highly on granulocytes and weakly on monocytes in peripheral blood, and plays a role in cell adhesion. It has been identified as a key adhesive ligand that plays a significant role in the extravascular infiltration of cells associated with acute myeloid leukemia (AML).
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- THP-1 cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
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Application References
(PubMed link indicates BioLegend citation) -
- Lund-Johansen F, et al. 1992. J Immunol. 148:3221-9.
- Bordessoule D, et al. 1993. Br J Haematol. 83:370-83.
- Kniep B, et al. 1996. J. Biochem 119:456-62.
Antigen Details
- Structure
- The structure of CD65 is defined as Gal beta1-4 GlcNAc beta1-3 Gal beta1-4 GlcNAc (3-1 Fuc alpha) beta1-3 ceramide.
- Distribution
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Most myeloid cells during development, highly on granulocytes and weakly on monocytes in peripheral blood.
- Function
- Cell adhesion
- Ligand/Receptor
- Serve as a ligand for CD62E, also known as E-selection
- Cell Type
- Granulocytes, Leukocytes, Monocytes
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules
- Antigen References
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- Joziasse DH, et al. 1989. J. Biol. Chem. 264:14290-7.
- Gene ID
- NA
Other Formats
View All CD65 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD65 Antibody | VIM8 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD65 Antibody
Human peripheral blood leukocytes were stained with purified...
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