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Purified anti-human CD66a/b/c/e Antibody

Pricing & Availability
Clone
5B2 (See other available formats)
Regulatory Status
RUO
Other Names
CEACAM1,5,6,8
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
5B2_PURE_CD66a-b-c-e_Antibody_1_111519.png
Human paraffin-embedded colon tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citric buffer 1x pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD66a/b/c/e (clone 5B2) antibody overnight at 4°C. On the next day, tissue was incubate with Alexa Fluor® 594 goat anti-mouse IgG (clone poly4053) antibody (red). Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
  • 5B2_PURE_CD66a-b-c-e_Antibody_1_111519.png
    Human paraffin-embedded colon tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citric buffer 1x pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD66a/b/c/e (clone 5B2) antibody overnight at 4°C. On the next day, tissue was incubate with Alexa Fluor® 594 goat anti-mouse IgG (clone poly4053) antibody (red). Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
  • 5B2_PURE_CD66a-b-c-e_Antibody_2_111519.png
    Human paraffin-embedded spleen tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citric buffer 1x pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD66a/b/c/e (clone 5B2) antibody overnight at 4°C. On the next day, tissue was incubate with Alexa Fluor® 594 goat anti-mouse IgG (clone poly4053) antibody (red). Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
  • 5B2_PURE_CD66a-b-c-e_Antibody_3_022520_updated
    Human colorectal adenocarcinoma cell line HT-29 stained with CD66a/b/c/e (clone 5B2) purified (filled histogram) or mouse IgG1, κ isotype control (clone MOPC-21) purified (open histogram) followed by anti-mouse IgG PE.
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398902 100 µg £97
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Description

The 5B2 monoclonal antibody reacts with the antigen epitopes shared by CD66a, b, c and e, which are members of the CEA (carcinoembryonic antigen) family of the Ig superfamily. CD66 plays a role in hemophilic and heterophilic adhesion. CD66a, also known as CEACAM1 and BGP (biliary glycoprotein), is mainly expressed on granulocytes, binds to CD66a, c, e, and CD62E. CD66c (known as CEACAM6, NCA) is expressed on granulocytes and epithelial cells. The ligands of CD66c are CD66a-e, CD62E and Galectins. CD66d is a major granulocyte receptor mediating recognition and efficient opsonin-independent phagocytosis of CEACAM-binding microorganisms, including Neisseria, Moraxella and Haemophilus species, thus playing an important role in the clearance of pathogens by the innate immune system. CD66e molecule is also called CEA or CEACAM5, and is primarily found on epithelial cells. CD66e binds to CD66a, c and e.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
FC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2820072 (BioLegend Cat. No. 398902)

Antigen Details

Structure
Ig superfamily, CEA antigen group, GPI-linked glycoprotein
Distribution

Granulocytes, epithelial cells

Function
Homophilic and heterophilic adhesion
Ligand/Receptor
CD66a-e, CD62E, galectins
Cell Type
Epithelial cells, Granulocytes
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiley-Liss. A John Wiley & Sons Inc. Publication
  2. Chen T, et al. 2001. J Biol Chem. 276:17413.
  3. Wang Z, et al. 2016. Mol Clin Oncol. 5:731.
  4. Swords DS, et al. 2016. Onco Targets Ther. 9:7459
Gene ID
1088 View all products for this Gene ID 634 View all products for this Gene ID 4680 View all products for this Gene ID 1048 View all products for this Gene ID
UniProt
View information about CD66a/b/c/e on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 02/25/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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