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Purified anti-human CD68 Antibody

Pricing & Availability
Clone
Y1/82A (See other available formats)
Regulatory Status
RUO
Workshop
VI MR23
Other Names
Macrosialin
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A_Y1slash82A_Pure_070808.jpg
Human peripheral blood monocytes intracellularly stained with purified Y1/82A, followed by anti-mouse IgG FITC
  • A_Y1slash82A_Pure_070808.jpg
    Human peripheral blood monocytes intracellularly stained with purified Y1/82A, followed by anti-mouse IgG FITC
  • B_Y182A_Purified_CD68_Antibody_IF_112012
    MDA-MB-231 breast cancer cells were stained with anti-human CD68 (clone Y1/82A) using 1:100 dilution, followed by DyLight™ 649 anti-mouse Ig secondary antibody (red) plus DAPI staining for nuclei (blue). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, blocked with 10% serum, and incubated O/N at 4°C. Images were taken under 20x (filter set: EX 647/10x, Dichroic 665LP, EM 700/70x) at exposure 4s. Data provided by John Nolan and Er Liu, La Jolla Bioengineering Institute.
Compare all formats
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333801 25 µg £45
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333802 100 µg £78
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Description

CD68 is a 110 kD glycoprotein, also known as macrosialin, belonging to the sialomucin family. It is closely related to the family of acidic, highly glycosylated lysosomal-associated membrane proteins (LAMPs). CD68 is predominately expressed in cytoplasmic granules of monocytes/macrophages, dendritic cells, and granulocytes. It is one of the useful myeloid cell markers. Further studies have shown that CD68 is also expressed by a subset of hematopoietic progenitors, γ/δ T cells, NK cells, LAK cells, subset of B cells, fibroblasts, and endothelial cells. The biological function of CD68 is still unknown.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
ICC - Verified
IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported application: immunohistochemical staining of frozen tissue sections. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

Application References

(PubMed link indicates BioLegend citation)
  1. Doussis IA, et al. 1993. J. Clin. Pathol. 46:334.
  2. Davey FR, et al. 1988. J. Clin. Pathol. 41:753.
  3. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
Product Citations
  1. Rajagopalan A, et al. 2021. Cell Rep Methods. 1:. PubMed
  2. Aly NAR, et al. 2023. Front Oncol. 12:1009993. PubMed
  3. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
  4. Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
  5. Zhang XN, et al. 2021. Cell Res. . PubMed
  6. Carpenter J, et al. 2011. J Virol. 85:9414. PubMed
  7. Wei SC et al. 2017. Cell. 170(6):1120-1133 . PubMed
  8. Nugent JL, et al. 2021. iScience. 24:103421. PubMed
  9. Feng Q, et al. 2017. J Thromb Haemost. 1.90625. PubMed
  10. Greven D, et al. 2015. Ann Rheum Dis. 74: 1924 - 1930. PubMed
  11. Talbot H, et al. 2020. Sci Rep. 10:12572. PubMed
  12. Cole JM, et al. 2021. Cancers (Basel). 13:00. PubMed
  13. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
  14. Trumpi K, et al. 2018. Oncotarget. 3. PubMed
  15. Chevrier S, et al. 2018. Cell Syst. 0.675. PubMed
  16. Scharenberg SG, et al. 2020. Nat Commun. 2.76875. PubMed
  17. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
RRID
AB_1089058 (BioLegend Cat. No. 333801)
AB_1089058 (BioLegend Cat. No. 333802)

Antigen Details

Structure
Sialomucin family, 110 kD
Distribution

Monocytes/macrophages, dendritic cells, granulocytes, subset of hematopoietic progenitors, γ/δ T cells, NK cells, LAK cells, subset of B cells, fibroblasts, endothelial cells

Cell Type
B cells, Dendritic cells, Endothelial cells, Fibroblasts, Granulocytes, Hematopoietic stem and progenitors, Macrophages, Monocytes, T cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Holness CL and Simmons DL. 1993. Blood 81:1607.
2. Gottfried E, et al. 2008. Scand. J. Immunol. 67:453.
3. Hameed A, et al. 1994. Hum. Pathol. 25:872.

Gene ID
968 View all products for this Gene ID
UniProt
View information about CD68 on UniProt.org
Go To Top Version: 2    Revision Date: 01/20/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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