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Purified anti-human CD98 Antibody

Pricing & Availability
Clone
MEM-108 (See other available formats)
Regulatory Status
RUO
Other Names
4F2, FRP-1, RL-388
Isotype
Mouse IgG1, κ
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Product Citations
publications
MEM-108_Purified_070507
Human peripheral blood lymphocytes stained with purified MEM-108, followed by anti-mouse IgGs FITC
  • MEM-108_Purified_070507
    Human peripheral blood lymphocytes stained with purified MEM-108, followed by anti-mouse IgGs FITC
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315602 100 µg £77
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Description

CD98 is a 125 kD disulfide-linked heterodimer protein also known as 4F2 and FRP-1. It is broadly expressed on activated and transformed cells (hematopoietic and non-hematopoietic), and at low levels on quiescent cells. CD98 has been characterized as a potential amino acid transporter (neutral and dibasic amino acids) that is involved in lymphocyte activation and integrin signaling. CD98 is composed of a light chain (45 kD) and a heavy chain (80 kD). Studies with knock-out mice indicate that the CD98 heavy chain is involved in integrin-dependent cell spreading and protection against anchorage deprivation-induced apoptosis. The MEM-108 antibody is useful for flow cytometry and immunoprecipitation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Burkitt's lymphoma cell line Raji
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation.

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. New York.
  2. Cantor J, et al. 2011. J. Immunol. 187:851. PubMed.
Product Citations
  1. Weng L, et al. 2018. PLoS Biol. 7:e1428158. PubMed
  2. Dhawan K, et al. 2022. Mol Biol Cell. 33:ar107. PubMed
  3. Feliciano D, et al. 2021. Nat Commun. 12:4502. PubMed
  4. Wunderley L, et al. 2021. J Cell Sci. . PubMed
  5. Wunderley L, et al. 2021. J Cell Sci. 134:. PubMed
  6. Higashi S, et al. 2022. J Cell Sci. 135:. PubMed
  7. Arndt C, et al. 2020. Oncoimmunology. 9:1743036. PubMed
  8. Cantor J, et al. 2011. J Immunol. 187:851. PubMed
RRID
AB_2302070 (BioLegend Cat. No. 315602)

Antigen Details

Structure
Disulfide-linked heterodimer, 80 kD and 45 kD reduced, 125 kD unreduced
Distribution

Broadly expressed on activated and transformed cells, not hematopoietic cell-specific. Lower levels on resting cells

Function
Potential amino acid transporter (neutral and dibasic amino acids) also involved in the activation of lymphocytes and integrin signaling. May be a target antigen for NK cells
Interaction
Actin, β1 integrins (α2β1, α3β1, α5β1, α6β1; minimally with α4β1)
Modification
Glycosylated (heavy chain)
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Leukocyte Typing VI. Kishimoto T, et al. (Eds.) Garland Publishing Inc. (1997)
2. Bron C, et al. 1986. J. Immunol. 137:397.
3. Lumadue JA, et al. 1987. Proc. Natl. Acad. Sci. 84:9204.
4. Mastroberardino L, et al. 1998. Nature 395:288.

Gene ID
6520 View all products for this Gene ID
UniProt
View information about CD98 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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