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Purified anti-human CLEC1B (CLEC2) Antibody

Pricing & Availability
Clone
AYP1 (See other available formats)
Regulatory Status
RUO
Other Names
C-type lectin domain family 1 member B, CLEC2B, PRO1384, QDED721
Isotype
Mouse IgG1, κ
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Product Citations
publications
AYP1_PURE_CLEC1B_Antibody_FC_100616
Human platelets were stained with purified CLEC1B (clone AYP1, filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • AYP1_PURE_CLEC1B_Antibody_FC_100616
    Human platelets were stained with purified CLEC1B (clone AYP1, filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
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372002 100 µg £221
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Description

C-type lectin domain family 1 member B (CLEC1B, CLEC2) is a 32kD, type II transmembrane protein, highly expressed on platelets and megakaryocytes. CLEC1B is involved in platelet activation and aggregation, and mediates the capture of HIV-1 by platelets. CLEC1B contains one hem-immunoreceptor tyrosine-based activation motif (hemITAM) though which interact with Syk and PLC-g. The known ligands of CLEC1B are rhodocytin and podoplanin.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Extracellular region of CLEC1B (amino acids 68-229).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation; inhibition of podoplanin and rhodocytin-mediated platelet activation.

Application References

(PubMed link indicates BioLegend citation)
  1. Gitz E, et al. 2014. Blood 124:2262.
RRID
AB_2632624 (BioLegend Cat. No. 372002)

Antigen Details

Structure
Type II transmembrane protein, 32 kD, homodimer, 1 hemITAM motif.
Distribution

Platelets, megakaryocytes; low expression on myeloid cells.

Function
Mediates platelet activation and aggregation; facilitates capture of HIV by platelets.
Interaction
Syk, PLC-g.
Ligand/Receptor
Rhodocytin, podoplanin.
Cell Type
Dendritic cells, Megakaryocytes, Platelets
Biology Area
Immunology
Antigen References

1. Manne BK, et al. 2015. J. Biol. Chem. 290:11557.
2. Hughes CE, et al. 2013. J. Biol. Chem. 288:5127.
3. Parguiña AF, et al. 2012. Blood 120:e117-26.
4. Osada M, et al. 2012. J. Biol. Chem. 287:22241.

Gene ID
51266 View all products for this Gene ID
UniProt
View information about CLEC1B on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/07/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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