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Purified anti-human CXCL16 Antibody

Pricing & Availability
Clone
22-19-12 (See other available formats)
Regulatory Status
RUO
Other Names
SRPSOX, CXCXLG16, SR-PSOX
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
22-19-12_Purified_CXCL16_Antibody_FC_111213
Peripheral blood monocytes were stained with purified anti-human CXCL16 (clone 22-19-12, filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • 22-19-12_Purified_CXCL16_Antibody_FC_111213
    Peripheral blood monocytes were stained with purified anti-human CXCL16 (clone 22-19-12, filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
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360802 100 µg £157
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Description

CXCL16, a 27 kD chemokine, exists in membrane-bound and soluble forms.  Unlike classic chemokines, CXCL16 possesses a mucin-like stalk, a transmembrane domain, and a cytoplasmic tail with a tyrosine phosphorylation domain, allowing it to be expressed on the cell surface.  Structurally, it is similar to fractalkine.  It is expressed by dendritic cells, monocytes, and T cells.  T cells can also migrate in response to CXCL16.

CXCL16 can also act as a scavenger receptor on macrophages. Its receptor is CXCR6/Bonzo.  It can bind oxidized LDL and thus may play an important role in atherosclerosis.  Recent studies have shown a role for CXCL16 in kidney fibrosis and in autoimmunity.  CXCL16 is also a co-receptor for HIV.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified human SR-PSOX-Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats of this clone) include: in vitro blocking of chemotactic activity of CXCR6 expressing cells1. Clone 22-19-12 recognizes the chemokine domain of CXCL16 and has been shown to inhibit chemotactic activity to CXCR6-expressing cells by soluble CXCL16.1

Application References

(PubMed link indicates BioLegend citation)
  1. Shimaoka T, et al. 2003. J. Immunol. 171:1647. (FC, Block)
RRID
AB_2562794 (BioLegend Cat. No. 360802)

Antigen Details

Structure
~27 kD, exists as a membrane bound and soluble forms
Distribution
Produced by dendritic cells and T cells
Function
Binds to oxidized LDL and may play an important role in atherosclerosis
Interaction
Interacts with CXCR6/Bonzo
Ligand/Receptor
CXCR6
Cell Type
Dendritic cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Matloubian M, et al. 2000. Nat. Immunol. 1:298.
2. Wilbanks A, et al. 2001. J. Immunol. 166:5145.
3. Shimaoka T, et al. 2003. J. Immuniol. 171:1647.
4. Chandrasekar B, et al. 2004. J. Biol. Chem. 179:3188.
5. Shimaoka T, et al. 2004. J. Leukoc. Biol. 75:267.

Gene ID
58191 View all products for this Gene ID
UniProt
View information about CXCL16 on UniProt.org
Go To Top Version: 2    Revision Date: 08/21/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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