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Purified anti-human CXCL5 (ENA-78) Antibody

Pricing & Availability
Clone
J1119G12 (See other available formats)
Regulatory Status
RUO
Other Names
ENA-78
Isotype
Mouse IgG1, λ
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CXCL5_Human_RECOM_ELISAPAIR_072214.jpg
  • CXCL5_Human_RECOM_ELISAPAIR_072214.jpg
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526201 50 µg £101
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526202 500 µg £245
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Description

CXCL5 is a member of the CXC family of chemokines, also known as epithelial activated peptide 78 (ENA-78). The precursor of CXCL5 contains 114 amino acids, with a signal peptide of 36 amino acids which is cleaved to generate the 78 amino acid mature protein (8 kD). CXCL5 is expressed by monocytes/macrophages, endothelial cells, vascular smooth muscle cells, epithilial cells, and eosinophils. CXCL5 is an inflammatory cytokine which binds to the cell surface receptor CXCR2 and induces chemotaxis of neutrophils to a target site. High levels of CXCL5 have been associated with prostate, gastric, and pancreatic cancer progression.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Mouse CXCL5
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. A concentration of 1.5 µg/ml of the capture antibody was utilized to generate the example standard curve. It is recommended that the end-user titrate each lot of reagents for optimal performance.

Application Notes

ELISA Capture: To measure human CXCL5, this antibody can be used as a capture antibody in sandwich ELISA format and paired with the biotinylated J1119D2 antibody (Cat. No. 526304) as the detection antibody. Recombinant Human CXCL5 (ELISA Std.) (Cat. No. 573409) can be used as the protein standard.
Note: For measuring human CXCL5 in serum, plasma or cell culture supernatant, LEGEND MAX™ Human CXCL5 ELISA Kit with Pre-coated Plates (Cat. No. 440907 & 440908) is specially developed and recommended.

RRID
AB_2563676 (BioLegend Cat. No. 526201)
AB_2563676 (BioLegend Cat. No. 526202)

Antigen Details

Structure
Chemokine
Distribution
Endothelial cells, fibroblasts, neutrophils, monocytes, macrophages, platelets, alveolar type II cells
Function
CXCL5 chemoattracts and activates neutrophils during inflammation, and it is induced by LPS and inflammatory cytokines. NH2-terminal truncation enhances, whereas citrullination decreases the chemotactic properties of CXCL5.
Interaction
Neutrophils
Ligand/Receptor
CXCR2
Cell Type
Endothelial cells, Fibroblasts, Neutrophils, Monocytes, Macrophages, Platelets, Eosinophils
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Walz A, et al. 1991. J. Exp. Med. 174:1355.
2. Wuyts A, et al. 1999. J. Immunol. 163:6155.
3. Jeyaseelan S, et al. 2005. Am. J. Respir. Cell. Mol. Biol. 32:531.
4. Mei J, et al. 2010. Immunity 33:106.
5. Mortier A, et al. 2010. J. Biol. Chem. 285:29750.
6. Grommes J and Soehnlein O. 2011. Mol. Med. 17:293.
7. Liu Y, et al. 2011. J. Immunol. 186:3197.
8. Kuo PL, et al. 2011. J. Cell. Physiol. 226:1224.
9. Power CA, et al. 1994. Gene. 151:333.
10. Corbett MS, et al. 1994. Biochem. Biophys. Res. Commun. 205:612.

Gene ID
6374 View all products for this Gene ID
UniProt
View information about CXCL5 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/22/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human CXCL5 (ENA-78)

    CXCL5_Human_RECOM_ELISAPAIR_072214.jpg
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