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Purified anti-human DKK-1 Antibody

Pricing & Availability
Clone
W19277H (See other available formats)
Regulatory Status
RUO
Other Names
Dickkopf-related protein 1, DKK1, Dickkopf WNT signaling pathway inhibitor 1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W19277H_PURE_DKK-1_Direct-ELISA_Antibody_052323
Direct ELISA showing purified anti-human DKK-1 (clone W19277H) bound to plate-immobilized recombinant human DKK-1 protein (Cat. Nos. 778602, 778604, and 778606). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP goat anti-rat IgG (Cat. No. 405405). TMB substrate (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
  • W19277H_PURE_DKK-1_Direct-ELISA_Antibody_052323
    Direct ELISA showing purified anti-human DKK-1 (clone W19277H) bound to plate-immobilized recombinant human DKK-1 protein (Cat. Nos. 778602, 778604, and 778606). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP goat anti-rat IgG (Cat. No. 405405). TMB substrate (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
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617052 100 µg £305
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Description

DKK-1 (Dickkopf related protein 1) is a member of the Dickkopf family of proteins that include DKK-1, -2, -3, -4, and Soggy. DKK-1 was discovered by its ability to block Wnt signaling during early Xenopus embryogenesis. DKK-1 antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt proteins. DKK-1 forms a ternary complex with LRP5/6 and the transmembrane protein Kremen1/2, promoting internalization of LRP5/6 and down regulation of Wnt signaling. DKK-1 contains two conserved cysteine-rich domains separated by a linker region. The N-terminal cysteine-rich domain is unique to DKKs while the C-terminal cysteine-rich domain has a pattern of 10 cysteines similar to colipase. DKK-1 binds with LRP6 with high affinity and this interaction is enhanced by Kremens. The interactions between DKK-1 and both LRP6 and Kremens are mediated via C-terminal cysteine-rich domain. DKKs are expressed throughout development and play an important role by locally inhibiting Wnt regulated processes such as limb development, somitogenesis, anteroposterior axial patterning, and eye formation. In adults, DKKs are implicated in bone formation and bone disease, cancer and Alzheimer's disease. The balance between Wnt signaling and DKK-1 inhibition is important for bone homeostasis. Increased expression of DKK-1 are found in lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers. DKK-1 expression is decreased in malignant melanoma and colorectal cancers.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human Recombinant DKK-1 Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

Direct ELISA - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.

Additional Product Notes

This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone W19277H) could function as a detection antibody when paired with purified anti-human DKK-1 antibody (Cat. No. 616552) (clone W19278B) as the capture antibody and using Recombinant Human DKK1 protein (Cat. Nos. 778602, 778604, and 778606) as the assay standard. For assay development flexibility purpose this product is provided as purified format.

Antigen Details

Distribution

Placenta, prostate, colon, spleen, and platelets.

Function
Wnt antagonist implicated in bone formation and bone disease, cancer and Alzheimer's disease. Also promotes type 2 cell-mediated inflammation.
Interaction
Bone
Ligand/Receptor
LRP5/6, Kremen-1, and Kremen-2
Cell Type
Embryonic Stem Cells
Biology Area
Cancer Biomarkers, Neuroinflammation, Neuroscience, Stem Cells
Antigen References
  1. Fedi P, et al. 1999. J Biol Chem. 274:19465-72.
  2. Krupnik VE, et al. 1999. Gene. 238:301-13.
  3. Mao B, et al. 2001. Nature. 411:321-5.
  4. Mukhopadhyay M, et al. 2001. Dev Cell. 1:423-34.
  5. Tian E, et al. 2003. New Eng J Med. 349:2483-94. 
  6. Niehrs C. 2006. Oncogene. 25:7469-81.
  7. Diarra D, et al. 2007. Nature Med. 12:156-163.
  8. Fujita K and Janz S. 2007. Molecular Cancer. 6:71.
  9. Ahn VE, et al. 2011. Dev Cell. 21:862-73.
Gene ID
22943 View all products for this Gene ID
UniProt
View information about DKK-1 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06/13/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

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