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Purified anti-human EGFR Antibody

Pricing & Availability
Clone
AY13 (See other available formats)
Regulatory Status
RUO
Other Names
Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, HER1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
AY13_PURE_EGFR_Antibody_1_FC_083016
HELA cells stained with purified EGFR (clone AY13, filled histogram) or purified mouse lgG1, K isotype control (open histogram), followed by anti-mouse lgG PE.
  • AY13_PURE_EGFR_Antibody_1_FC_083016
    HELA cells stained with purified EGFR (clone AY13, filled histogram) or purified mouse lgG1, K isotype control (open histogram), followed by anti-mouse lgG PE.
  • AY13_PURE_EGFR_Antibody_2_ICC_062320
    HeLa cells were fixed with 2% PFA for ten minutes, permeabilized with 0.5% Triton X-100 for five minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2 µg/mL anti-human EGFR Antibody (clone AY13) followed by Alexa Fluor® 594 (red) conjugated goat anti-mouse IgG in blocking buffer for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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352901 25 µg £61
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352902 100 µg £165
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Description

Epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein and member of the protein kinase superfamily that regulates cell growth and differentiation. EGFR binds EGF, TGF-α, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30, and vaccinia virus growth factor - all members of the EGF family. Ligand binding induces EGFR dimerization and autophosphorylation, initiating the MAPK, Akt, and JNK signaling pathways. EGFR is expressed by epithelial and endothelial cells and is frequently expressed by epithelial carcinomas.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Non-small cell lung cancer (NSCLC) cell line NCI-H322
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunocytochemistry, the suggested use of this reagent is 1.0 - 5.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Yamaguchi M, et al. 2009. The 15th Annual Meeting Japan Society of Gene Therapy. p1056. Abstract 92.
Product Citations
  1. Chen Z, et al. 2020. Glia. 68:2148. PubMed
  2. Leelatian N, et al. 2020. eLife. 9:e56879.. PubMed
  3. Hutt M, et al. 2018. Oncotarget. 9:11322. PubMed
  4. Han X, et al. 2017. Mol Ther. 25(1):274-284. PubMed
  5. Senosain MF, et al. 2021. Sci Rep. 11:14424. PubMed
  6. Ghatge M, et al. 2018. Mol Med Rep. 17:4253. PubMed
RRID
AB_10916396 (BioLegend Cat. No. 352901)
AB_10916396 (BioLegend Cat. No. 352902)

Antigen Details

Structure
Member of the protein kinase superfamily; transmembrane glycoprotein; ligand binding induces dimerization and autophosphorylation
Distribution

Epithelial cells and endothelial cells; frequently found in epithelial carcinomas

Function
Controls cell growth and differentiation
Interaction
MAPK, Akt, JNK
Ligand/Receptor
Members of the epidermal growth factor (EGF) family such as EGF, TGF-α, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor
Cell Type
Endothelial cells, Epithelial cells
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family
CD Molecules, Growth Factors
Antigen References

1. da Cunha Santos G, et al. 2011. Annu. Rev. Pathol. 6:49.
2. Gusterson BA and Hunter KD. 2009. Lancet Oncol. 10:522.
3. Mano M and Humblet Y. 2008. Nat. Clin. Pract. Oncol. 5:415.
4. Pao W and Chmielecki J. 2010. Nat. Rev. Cancer 10:760.

Gene ID
1956 View all products for this Gene ID
UniProt
View information about EGFR on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 06/23/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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