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Purified anti-human G-CSF Antibody

Pricing & Availability
Clone
A20052Q (See other available formats)
Regulatory Status
RUO
Other Names
Granulocyte-Colony Stimulating Factor, CSF3, CSF-β
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A20052Q_PURE_-G-CSF_Antibody_052723
LPS-stimulated (O/N, left) human peripheral blood mononuclear cells (in the presence of monensin) or unstimulated human peripheral blood mononuclear cells (right) were fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer (Cat. No. 426803) and then intracellularly stained with purified anti-human G-CSF (clone A20052Q) followed by PE anti-rat IgG. The cells were stained with anti-human CD14 APC. Data shown was from gated monocyte population.
  • A20052Q_PURE_-G-CSF_Antibody_052723
    LPS-stimulated (O/N, left) human peripheral blood mononuclear cells (in the presence of monensin) or unstimulated human peripheral blood mononuclear cells (right) were fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer (Cat. No. 426803) and then intracellularly stained with purified anti-human G-CSF (clone A20052Q) followed by PE anti-rat IgG. The cells were stained with anti-human CD14 APC. Data shown was from gated monocyte population.
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387602 100 µg £264
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Description

Human G-CSF was cloned from a human squamous carcinoma cell line and is the key hematopoietic cytokine involved in the control of neutrophil production. Therefore, it is a critical regulator of innate immunity against bacterial infections. G-CSF mobilizes stem cells indirectly by downregulating the expression of CXCL12 on marrow osteoblasts and by releasing neutrophil and monocyte proteolytic enzymes, including neutrophil elastase, cathepsin G, and matrix metalloproteinase-9, which in turn degrades important HSC trafficking and adhesion molecules c-kit, VCAM-1, and CXCR4. In addition, G-CSF possesses immunosuppressive effects on monocytes/macrophages, dendritic cells, and T lymphocytes. The receptor for G-CSF is expressed not only in hematopoietic cells; it is also expressed in cardiomyocytes, skeletal muscle, and neurons. G-CSF influences mouse skeletal muscle development and regeneration by stimulating myoblast proliferation. In vitro, G-CSF displays strong antiapoptotic activity in neuronal cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant Human G-CSF
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clones A20052D and A20052Q do not block each other.

RRID
AB_2941576 (BioLegend Cat. No. 387602)

Antigen Details

Structure
Heterodimer
Distribution

Macrophages, monocytes, neutrophils, fibroblasts, endothelial cells, epithelial cells, and bone marrow stromal cells

Function
G-CSF acts on myeloid progenitor cells to stimulate survival, proliferation, and neutrophilic maturation; on mature neutrophils, G-CSF enhances survival, superoxido anion and alkaline phosphatase production, arachidonic acid release, and antibody dependent cellular cytotoxicity.
Interaction
Myeloid progenitor cells
Ligand/Receptor
CD114 (G-CSFR)
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Nagata S, et al. 1986. Nature. 319:415-8.
  2. Kim HK, et al. 2006. Blood. 108:812-20.
  3. Donahue RE, et al. 2009. Blood. 114:2530-41.
  4. Shimoji K, et al. 2010. Cell Stem Cell. 6:227-37. 
  5. Martins A, et al. 2010. IUBMB Life. 62:611-7.
  6. Hara M, et al. 2011. J Exp Med. 208:715-27.
Gene ID
1441 View all products for this Gene ID
UniProt
View information about G-CSF on UniProt.org
Go To Top Version: 1    Revision Date: 05/26/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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