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Purified anti-human GM-CSF Antibody

Pricing & Availability
Clone
BVD2-23B6 (See other available formats)
Regulatory Status
RUO
Other Names
Granulocyte/macrophage colony-stimulating factor, CSF-α, Pluripoietin-α, Eosinophil colony stimulating factor (Eo-CSF), Burst promoting activity (BPA)
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
Human_GM-CSF_020405
  • Human_GM-CSF_020405
Cat # Size Price Quantity Check Availability Save
502201 50 µg £73
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502202 500 µg £95
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Description

Granulocyte/macrophage - colony stimulating factor (GM-CSF) is a hematopoietic factor that is produced by activated T cells, B cells, mast cells, macrophages, fibroblasts and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte and eosinophil progenitors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli -expressed, recombinant human GM-CSF
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
IP, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2-6 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of GM-CSF recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Capture1-6: The Purified BVD2-23B6 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated BVD2-21C11 antibody (Cat. No. 502304) as the detecting antibody. The Ultra-LEAF™ Purified antibody is suggested for ELISPOT capture.
Neutralization1-4: The Ultra-LEAF™ Purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human GM-CSF bioactivity (Cat. Nos. 502205 - 502210).
Additional reported applications (for the relevant formats) include: immunoprecipitation3 and Western blotting.
Note: For testing human GM-CSF in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. Nos. 432001 and 432004) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Bacchetta R, et al. 1990. J. Immunol. 144:902.
  2. Kita H, et al. 1991. J. Exp. Med. 174:745.
  3. Abrams J, et al. 1992. Immunol. Rev. 127:5.
  4. Abrams J, et al. 1994. Eosinophils in Allergy and Inflammation. Marcel Dekker New York. p.133.
  5. Mahanty S, et al. 1992. J. Immunol. 148:3567.
  6. Klinman D, et al. 1994. Curr. Prot. Immunol.. John Wiley and Sons New York. Unit 6.19.
Product Citations
  1. Matsumoto T, et al. 2015. J Asthma Allergy. 29:8. PubMed
  2. Takeuchi S, et al. 2015. Cancer Res . 75: 2629 - 2640. PubMed
RRID
AB_315211 (BioLegend Cat. No. 502201)
AB_315211 (BioLegend Cat. No. 502202)

Antigen Details

Structure
Cytokine; 22 kD (Mammalian)
Bioactivity
Growth/development granulocyte/macrophage progenitors; differentiates myeloblasts/monoblasts; synergizes with Epo proliferation of erythroid/megakaryocytic progenitors
Cell Sources
T cells, macrophages, fibroblasts, endothelial cells, mast cells
Cell Targets
Granulocyte/macrophage/erythroid/megakaryocytic progenitors, myeloblasts, monoblasts
Receptors
Heterodimer GM-CSFR α subunit (CDw116); β-subunit (CDw131) which is also shared the IL-3 and IL-5 receptor α chains
Biology Area
Cell Biology, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Demetri G, et al. 1991. Blood 78:2791.
3. Fan D, et al. 1991. In vivo 5:571.
4. Negrin R, et al. 1992. Adv. Pharmacol. 23:263.

Regulation
Synergistic with IL-1, IL-3, G-CSF; E21R competitive antagonist for receptor binding; stored in ECM with heparan sulfate proteoglycans
Gene ID
1437 View all products for this Gene ID
UniProt
View information about GM-CSF on UniProt.org

Other Formats

View All GM-CSF Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human GM-CSF BVD2-23B6 ELISA Capture,IP,WB
Ultra-LEAF™ Purified anti-human GM-CSF BVD2-23B6 ELISA Capture,ELISPOT Capture,IP,Neut,WB
Go To Top Version: 2    Revision Date: 05/23/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human GM-CSF

    Human_GM-CSF_020405

  • Ultra-LEAF™ Purified anti-human GM-CSF

    HumanGM-CSF_061604
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