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Purified anti-human HGF Antibody

Pricing & Availability
Clone
W19372J (See other available formats)
Regulatory Status
RUO
Other Names
Scatter Factor (SF), Hepatopoietin (HPTA), Lung fibroblast-derived mitogen
Isotype
Mouse IgG2a, κ
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Product Citations
publications
W19372J_PURE_HGF_Antibody_041023
Direct ELISA showing purified anti-human HGF (clone W19372J) bound to plate-immobilized recombinant human HGF protein (Cat. No. 596402). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Goat anti-mouse IgG (Cat. No. 405306). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
  • W19372J_PURE_HGF_Antibody_041023
    Direct ELISA showing purified anti-human HGF (clone W19372J) bound to plate-immobilized recombinant human HGF protein (Cat. No. 596402). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Goat anti-mouse IgG (Cat. No. 405306). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
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613052 100 µg £161
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Description

HGF is a dimeric molecule composed of the α-subunit and β-subunit, linked by a disulfide bond. The C-terminus of the α-subunit is followed directly by the N-terminus of the β-subunit. HGF protects the epithelium, neurons, and cardiomyocytes during organ diseases. This protective effect is mediated through anti-apoptotic signals via inhibition of caspase-3 activity or induction of anti-apoptotic molecules, such as Bcl-xL. In addition, HGF prohibits Fas-mediated apoptosis signals via sequestration of Fas and Met receptor on cell surfaces. Also, HGF elicits the regression of fibrosis in numerous organs, such as scleroderma, cardiomyopathy, vocal scarring, and peritoneal fibrosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Recombinant HGF Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

Direct ELISA - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.

Additional Product Notes

This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone W19372J) could function as a capture antibody when paired with Biotin anti-human HGF antibody (Cat. No. 613153) (clone W19371A) as the detection antibody and using Recombinant Human HGF protein (Cat. No. 596401 or Cat. No. 596402) as the assay standard.

RRID
AB_2941610 (BioLegend Cat. No. 613052)

Antigen Details

Structure
Heterodimer (alpha and beta chains)
Distribution

Mesenchymal cells, liver sinusoidal endothelial, macrophages

Function
Stimulates epithelial cell proliferation, motility, morphogenesis, and angiogenesis. Hypoxia and hyperglycemia suppress HGF production. In addition, TGF-β and angiotensin-II depress HGF production in vitro.
Interaction
Hepathocytes, epithelial cells
Ligand/Receptor
MET receptor
Cell Type
Embryonic Stem Cells
Biology Area
Angiogenesis, Cell Biology, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References
  1. Kataoka H, et al. 2000. J Biol Chem. 275:40453-62.
  2. Wang X, et al. 2002. Mol Cell. 9:411-21.
  3. Wu MH, et al. 2004. Gene Ther. 11:170-80.
  4. Mizuno S, et al. 2008. Front Biosci. 13:7072-86.
  5. D'Angelo F, et al. 2013. Clin Exp Immunol. 174:60-72.
Gene ID
3082 View all products for this Gene ID
UniProt
View information about HGF on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/10/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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