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Purified anti-human IFN-β Antibody

Pricing & Availability
Clone
W20086B (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-β, Fibroblast interferon (Fi-IFN, F-IFN), Type-1 interferon, acid-stable interferon, R1-GI factor, IFN-b, IFN-beta
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
W20086B_PURE_IFN-b_Antibody_022024
Direct ELISA showing purified anti-human IFN-β (clone W20086B) bound to plate-immobilized recombinant human IFN-β protein. The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP goat anti-mouse IgG (Cat. No. 405306). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
  • W20086B_PURE_IFN-b_Antibody_022024
    Direct ELISA showing purified anti-human IFN-β (clone W20086B) bound to plate-immobilized recombinant human IFN-β protein. The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP goat anti-mouse IgG (Cat. No. 405306). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
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617552 100 µg £288
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Description

The IFN-β/W20086B clone recognizes natural human IFN-β and recombinant β1. It is capable of neutralizing IFN-β.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Recombinant IFN-β Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

Direct ELISA - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.

Additional Product Notes

This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone W20086B) could function as a detection antibody when paired with purified anti-human IFN-β (Cat. No. 631952) (clone W20086C) as the capture antibody and using Recombinant human IFN-β protein as the assay standard. For assay development flexibility purposes, this product is provided as a purified format.

RRID
AB_3662348 (BioLegend Cat. No. 617552)

Antigen Details

Structure
Interferon; contains 187 amino acids with predicted molecular weight 22 kD.
Distribution

Fibroblasts, epithelial cells

Interaction
Broad, includes NK cells, T suppressor cells, activated monocytes
Ligand/Receptor
IFNα/βR (IFNAR-1, IFNAR-2)
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Nagarajan U. 2011. Crit Rev Immunol. 31:459-74.
  2. Honda K, et al. 2005. Int Immunol. 17:1367-78.
Gene ID
3456 View all products for this Gene ID
UniProt
View information about IFN-beta on UniProt.org
Go To Top Version: 1    Revision Date: 02/22/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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