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Purified anti-human IL-22 Antibody

Pricing & Availability
Clone
A20056F (See other available formats)
Regulatory Status
RUO
Other Names
IL22, IL-D110, IL-TIF, ILTIF, MGC79382, TIFIL-23, TIFa, zcyto18
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A20056F_PURE_-IL-22_Direct-ELISA_Antibody_052323
Direct ELISA showing purified anti-human IL-22 (clone A20056F) bound to plate-immobilized recombinant human IL-22 protein (Cat. Nos. 571302, 571304, 571306, and 571308). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP goat anti-rat IgG (Cat. No. 405405). TMB substrate (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
  • A20056F_PURE_-IL-22_Direct-ELISA_Antibody_052323
    Direct ELISA showing purified anti-human IL-22 (clone A20056F) bound to plate-immobilized recombinant human IL-22 protein (Cat. Nos. 571302, 571304, 571306, and 571308). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP goat anti-rat IgG (Cat. No. 405405). TMB substrate (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
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617852 100 µg £182
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Description

IL-22 is a cytokine structurally related to IL-10. It was originally identified in mouse as a gene induced by IL-9 in T cells and mast cells. IL-22 was initially designated as ILTIF (IL-10-related T cell-derived inducible factor). IL-22 belongs to a family of cytokines with limited homology to IL-10, namely IL-10, IL-19, IL20, IL-24, IL-26, IL-28A, IL-28B and IL-29 (the later three also known as IFN-λ). Human IL-22 shares 79% amino acid identity with mouse IL-22 and 25% identity with human IL-10. IL-22 biological activity is initiated by the binding to a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which share sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). Both chains of the IL-22R complex belong to the class II CRF. Two types of IL-22 binding receptors have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 is produced by immune cells and acts on nonimmune cells to regulate local tissue inflammation. As a product of T helper 17 lineage of CD4 (+) effector lymphocytes, IL-22 plays a critical role in mucosal immunity as well as in deregulated inflammation observed in autoimmune diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human Recombinant IL-22 Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

Direct ELISA - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.

Additional Product Notes

This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone A20056F) could function as a capture antibody when paired with Biotin anti-human IL-22 antibody (Cat. No. 618052) (clone W20080F) as the detection antibody and using Recombinant Human IL-22 protein (Cat Nos. 571302, 571304, 571306, and 571308) as the assay standard.

RRID
AB_3083219 (BioLegend Cat. No. 617852)

Antigen Details

Structure
Cytokine
Distribution

Thymic lymphomas, T cells, activated T cells, macrophages, and mast cells

Function
Enhances the production of acute phase proteins in a hepatocyte cell line, HepG2; promotes Th17 cells to transmigrate through the blood-brain barrier in multiple sclerosis
Interaction
Interacts with a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains which are expressed in epithelial cells of inflammatory diseases such as psoriasis, Crohn's disease, rheumatoid arthritis, or allergic asthma
Ligand/Receptor
IL-22R1 and IL-10R2 receptor chains
Biology Area
Cell Biology, Immunology, Stem Cells
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Nagalakshmi ML, et al. 2004. Intern Immunopharmacol 4:679-91.
  2. Kebir H, et al. 2007. Nat Med. 13:1173-5.
  3. Gu Y, et al. 2008. Eur J Immunol. 38:1807-13.
  4. Pene J, et al. 2008. J Immunol. 180:7423-30.
  5. Dumotier L, et al. 2000. J immune. 164:1814-1819.
  6. Xie MH, et al. 2000. J Biol Chem. 765:31335-9.
  7. Jones BC, et al. 2008. Structure. 1333-44.
  8. Kotenko SV, et al. J Immunol. 166:7096-103.
  9. Chang C, et al. 2006. Cell Res 16:902-7. 
Gene ID
50616 View all products for this Gene ID
UniProt
View information about IL-22 on UniProt.org
Go To Top Version: 1    Revision Date: 06/14/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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