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Purified anti-human IL-31 Antibody

Pricing & Availability
Clone
Poly5300 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-31,
Isotype
Goat Polyclonal Ig
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Product Citations
publications
Poly5300_PURE_IL-31_Antibody_032717
This standard curve was generated at BioLegend for demonstration purposes only. Overnight incubation for standard and samples at 4°C is recommended. For detection antibody, two hour incubation with shaking is recommended.
  • Poly5300_PURE_IL-31_Antibody_032717
    This standard curve was generated at BioLegend for demonstration purposes only. Overnight incubation for standard and samples at 4°C is recommended. For detection antibody, two hour incubation with shaking is recommended.
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530002 100 µg £240
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Description

IL-31, produced mainly by activated CD4+ T cells, is a newly discovered helical cytokine belonging to the gp130/IL6 cytokine family. IL-31 is closely related in structure to IL-6, IL-11, and IL-27. Binding of IL-31 to its receptor activates JAK/STAT, PI3K/AKT, and MAPK pathways. IL-31 acts on a broad range of immune and non-immune cells and thus possesses potential pleiotropic physiological functions, such as regulating hematopoiesis and immune responses. Human IL-31 is also involved in inflammatory bowel disease, airway hypersensitivity, and dermatitis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant human IL-31
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by ELISA. For ELISA capture applications, a concentration of 1.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of IL-31 recombinant protein ranging from 2000 to 31.3 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance.

Application Notes

The purified Poly5300 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5300 antibody as the detecting antibody. 

RRID
AB_2650707 (BioLegend Cat. No. 530002)

Antigen Details

Structure
161 aa with a putative 23 aa signal peptide that is cleaved to generate a 138 aa mature protein.
Distribution

Secreted by activated CD4<sup>+</sup> T cells.

Function
IL-31 belongs to the IL-6 family of cytokines and plays a role in skin inflammation.
Ligand/Receptor
Binds to IL-31 receptor complex that consists of two receptor subunits, IL31RA and OSMR.
Cell Type
T cells
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. McCandless EE, et al. 2014. Vet. Immunol. Immunopathol. 157:42.
2. Arai I, et al. 2013. Exp. Dermatol. 22:669.
3. Dai X, et al. 2013. PLoS One. 8:e67666.
4. Stott B, et al. 2013. J. Allergy Clin. Immunol. 132:446.
5. Hartmann K, et al. 2013. J. Allergy Clin. Immunol. 132:232.
6. Baron JM and Lüscher B. 2012. Acta. Derm. Venereol. 92:5.

Gene ID
386653 View all products for this Gene ID
UniProt
View information about IL-31 on UniProt.org

Other Formats

View All IL-31 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human IL-31 Poly5300 ELISA Capture
Biotin anti-human IL-31 Poly5300 ELISA Detection
Go To Top Version: 0    Revision Date: 03/28/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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