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Purified anti-human IL-32αβδ Antibody

Pricing & Availability
Clone
KU32-56 (See other available formats)
Regulatory Status
RUO
Other Names
IL-32alpha,beta,delta, Interleukin 32 alpha,beta,delta, Natural Killer Transcript 4 (NK4), TNF-α-inducible factor (TAIFa,b,d)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
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513601 50 µg £94
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Description

Interleukin 32 (IL-32), previously known as a transcript (NK4), is produced by mitogen-activated lymphocytes, by IFNγ-activated epithelial cells or by IL-12 and IL-18-activated NK cells. Its expression is increased following activation of T-cells by mitogens or the activation of NK cells by IL-2. IL-32 activates NF-κB and p38 MAPK cytokine signal pathways. It has been suggested that IL-32 may play a role in autoimmune and inflammatory diseases such as rheumatoid arthritis. IL-32 is unusual in that it does not share sequence homology with known cytokine families and is highly expressed in immune tissues. IL-32 exists in at least four differentially spliced isoforms (α, β, γ and δ)with predicted molecular weight: ~26 kD. IL-32α is the shortest and most abundant of four potential splice variants of the pro-inflammatory cytokine IL-32. Potential modifications include myristoylation and N-glycosylation. Transfected IL-32 alpha was more likely to be cell-associated as compared to IL-32β, suggesting an intracellular function.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human IL-32α
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
WB, ICC, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA capture antibody, a concentration range of 4 - 6 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of human IL-32 recombinant protein ranging from 2000 to 15 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA1, 4 Capture: The purified KU32-56 antibody is useful as a capture antibody in a sandwich ELISA assay, when used in conjunction with biotinylated KU32-52 antibody as the detection antibody for measuring human IL-32.

Additional reported applications (for the relevant formats) include: Western blotting, immunofluorescence, and immunoprecipitation.

Clone KU32-56 is shown to be reactive towards IL-32 α, β, δ, but not the γ isoform and clone KU32-52 has been shown to bind to all isoforms of IL-32.1 Clone KU32-09 does not work for Western blotting.6

Application References

(PubMed link indicates BioLegend citation)
  1. Kim KH, et al. 2008. J. Immunol. Methods 333:38. (ELISA Capture)
  2. Kulkarni O,et al.2008. J Pharmacol Exp Ther. PubMed
  3. Greene CM, et al. 2010. AM J. Respir Crit Care Med. 181:31. PubMed
  4. Sakitani K, et al. 2012. Infect. Immun. 80:3795. (ELISA Capture) PubMed
  5. Li Y, et al. 2013. J Biol Chem. 288:20927. PubMed
  6. Seo Eun-hee, et al. 2008. J. Microbiol. Biotechnol. 9:1606-1612.
Product Citations
  1. Yun J, et al. 2018. Cell Death Dis. 3. PubMed
  2. Qu P, et al. 2021. J Autoimmun. 116:102559. PubMed
  3. CM G, et al. 2010. Am J Respir Crit Care Med. 2010181:31. PubMed
  4. Sakitani K, et al. 2012. Infect Immun. 80:3795. PubMed
  5. Li Y, et al. 2013. J Biol Chem. 288:20927. PubMed
  6. Kulkarni O, et al. 2009. J Pharmacol Exp Ther. 328:371. PubMed
RRID
AB_2248930 (BioLegend Cat. No. 513601)

Antigen Details

Structure
Cytokine
Distribution

Activated lymphocytes and NK cells

Cell Type
Lymphocytes, NK cells
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Kim KH, et al. 2008. J. Immunol. Methods 333:38.
2. Conti P, et al. 2007. Autoimmun. Rev. 6:131.
3. Chen Q, et al. 2006. Vitam Horm. 74:207.
4. Kim SH, et al. 2005. Immunity 22:131.
5. Cagnard N, et al. 2005. Eur. Cytokine Netw. 16:289.
6. Banda NK, et al. 2003. J. Immunol. 170:2100.

Gene ID
9235 View all products for this Gene ID
UniProt
View information about IL-32 on UniProt.org
Go To Top Version: 3    Revision Date: 01/19/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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  • Purified anti-human IL-32αβδ

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