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Purified anti-human IL-6 Antibody

Pricing & Availability
Clone
MQ2-39C3 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-6, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)
Isotype
Rat IgG2a, κ
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Product Citations
publications
HumanIL-6_061604
  • HumanIL-6_061604
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501204 500 µg £161
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Description

IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survival of certain B cells and T cells. IL-6 plays a role in host defense, acute phase reactions, immune response, and hematopoiesis. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells and keratinocytes. The MQ2-39C3 antibody can neutralize the bioactivity of natural or recombinant IL-6.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
COS-7- expressed, recombinant human IL-6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA, WB
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.5-2 µg/ml is recommended.
It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Detection1-4: The biotinylated MQ2-39C3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified MQ2-13A5 antibody (Cat. No. 501101/501102) as the capture antibody.
Additional reported applications (for the relevant formats) include: neutralization1, Western blotting. The LEAF™ purified MQ2-13A5 antibody (Endotoxin Note: For testing human IL-6 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430501 to 430506) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5.
  2. Abrams J. 1995. Curr. Prot. Immunol.. John Wiley and Sons New York. Unit 6.20.
  3. Gaines Das R, et al. 1993. J. Immunol. Meth. 160:147.
  4. Lesmeister MJ, et al. 2005. Reproductive Biology and Endocrinology 3:74.
Product Citations
  1. Rodriguez-Salazar CA, et al. 2022. Viruses. 14:. PubMed
RRID
AB_2296206 (BioLegend Cat. No. 501204)

Antigen Details

Structure
Neuropoietic cytokine family; 26 kD (Mammalian)
Bioactivity
Hematopoiesis; antigen-specific immune responses, inflammatory reactions; acute phase reaction
Cell Sources
T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells
Cell Targets
T cells, B cells, hepatocytes, cholinergic neurons
Receptors
Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130; gp130)
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Hirano T. 1998. Intl. Rev. Immunol. 16:249.
3. Patterson P. 1992. Curr. Opin. Neurobiol. 2:94.
4. Van Oers M, et al. 1993. Ann. Hematology 66:219.

Regulation
Upregulated by IL-1, PDGF, IFN-β, TNF-α, NGF, IL-17; downregulated by glucocorticoids IL-4, TGF-β
Gene ID
3569 View all products for this Gene ID
UniProt
View information about IL-6 on UniProt.org

Other Formats

View All IL-6 Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-human IL-6 MQ2-39C3 ELISA Detection,ELISPOT Detection,ICC
Purified anti-human IL-6 MQ2-39C3 ELISA,WB
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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