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Purified anti-human/mouse/rat CD205 (DEC-205) Antibody

Pricing & Availability
Clone
HD83 (See other available formats)
Regulatory Status
RUO
Other Names
LY75
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
HD83_Purified_CD205_Antibody_FC_1_080213.jpg
Human peripheral blood monocyte-derived dendritic cells were stained with CD209 (clone 9E9A8, mouse IgG2a) APC and purified CD205 (clone HD83) (top) or purified mouse IgG1, κ isotype control (bottom), followed by anti-mouse IgG1 PE.
  • HD83_Purified_CD205_Antibody_FC_1_080213.jpg
    Human peripheral blood monocyte-derived dendritic cells were stained with CD209 (clone 9E9A8, mouse IgG2a) APC and purified CD205 (clone HD83) (top) or purified mouse IgG1, κ isotype control (bottom), followed by anti-mouse IgG1 PE.
  • HD83_Purified_CD205_Antibody_FC_2_080213.jpg
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359202 100 µg £197
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Description

CD205, also known as DEC-205 and LY75, belongs to the macrophage mannose receptor (MMR) family of C-type lectins. Both human and mouse CD205 share multiple similar structures: an N-terminal cysteine-rich domain, a fibronectin type II domain, and a series of 10 tandemly repeated C-type like domains (CTLDs). CD205 is highly expressed on dendritic cells and thymic cortical epithelial cells in humans and mice. It also can be detected on T and B cells, monocytes, neutrophils, and epithelial cells. CD205 is a novel endocytic receptor used by dendritic cells and thymic epithelial cells to direct captured antigens from the extracellular space to specialized antigen processing areas. It mediates antigen uptake and presentation and cross-presentation to T cells. It has been reported that CD205 acts as a recognition receptor for dying cells, which potentially provides an important pathway for the uptake of self-antigen in the intrathymic environment, and is involved in peripheral tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Reported Reactivity
Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
hDEC205-hIgG1Fc protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for relevant formats) include: immunohistochemical staining of frozen tissue sections1 and Western blotting1.

Application References

(PubMed link indicates BioLegend citation)
  1. Park CG, et al. 2012. J. Immunol. Methods 377:15. (IHC, WB)
RRID
AB_2562362 (BioLegend Cat. No. 359202)

Antigen Details

Structure
An N-terminal cysteine-rich domain, a fibronectin type II domain and a series of 10 tandemnly repeated C-type like domains (CTLDs)
Distribution

Expressed at high levels by dendritic cells and thymic epithelial cells; also expressed by B lymphocytes, macrophages, Langerhans cells, bone marrow cells, and epithelial cells

Function
Mediates endocytosis, antigen uptake, presentation, and cross-presentation to T cells
Cell Type
B cells, Dendritic cells, Epithelial cells, Langerhans cells, Macrophages
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Jiang W, et al. 1995. Nature 375:151.
2. Small M and Kraal G. 2003. Int. Immunol. 15:197.
3. Shrimpton RE, et al. 2009. Mol. Immunol. 46:1229.

Gene ID
4065 View all products for this Gene ID 17076 View all products for this Gene ID 499800 View all products for this Gene ID
UniProt
View information about CD205 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/14/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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