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Purified anti-human Notch 4 Antibody

Pricing & Availability
Clone
MHN4-2 (See other available formats)
Regulatory Status
RUO
Other Names
Neurogenic locus notch homolog protein 4
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
MHN4-2_PE_070710
Human acute lymphoblastic leukemia cells HPB-ALL stained with purified MHN4-2 conjugated with PE
  • MHN4-2_PE_070710
    Human acute lymphoblastic leukemia cells HPB-ALL stained with purified MHN4-2 conjugated with PE
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349002 100 µg £182
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Description

Neurogenic locus notch homolog protein 4 (Notch 4) is a transmembrane heterodimer in which the extracellular domain contains 29 EGF repeats and 3 LNR repeats. The intracellular domain contains 5 ANK repeats, 1 PEST motif, and 1 RAM23 domain. Notch 4 controls many developmental processes in the cell such as differentiation, proliferation, and apoptosis. In addition, it also plays a role in vascular, renal, and hepatic development. Notch 4 is expressed by a variety of tissues such as heart, lung, placenta, liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow, and fetal liver. The Notch 4 ligands are Jagged1, Jagged2, Delta1, Delta3 and Delta4. After ligand binding, Notch 4 is cleaved by the TNF-α converting enzyme (TACE). The extracellular domain remains on the cell surface while the intracellular domain is released and translocated to the nucleus where it binds to CBF-1, leading to the expression of genes of the Hairy/Enhancer of Split (HES) and HES-related repressor protein (HERP) families.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Notch 4-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Kim H, et al. 2015. Cytotherapy. . PubMed
RRID
AB_10612568 (BioLegend Cat. No. 349002)

Antigen Details

Structure
Transmembrane protein. Extracellular domain forms a heterodimer and contains 29 epidermal growth factor-like repeats and 3 Lin/Notch Glp repeats. The intracellular domain contains 5 CDC10/Ankryn repeats, 1 proline, glutamate, serine, threonine-rich motif,
Distribution
Heart, lung, placenta, liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow, and fetal liver.
Function
Controls many cell developmental processes such as differentiation, proliferation and apoptosis. Plays a role in vascular, renal, and hepatic development.
Interaction
MAML1, MAML2 and MAML3, CBF-1, Smad3, and Smad4
Ligand/Receptor
Jagged1, Jagged2, Delta1, Delta3, and Delta4
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Transcription Factors
Antigen References

1. Katoh M and Katoh M. 2007. Int. J. Oncol. 30:247.
2. Kerbel RS. 2008. N. Engl. J. Med. 358:2039.
3. Chen VC, et al. 2008. Nat. Biotechnol. 26:1169.
4. Emuss V. 2009. PLoS Pathog. 5:e1000616.
5. Harrison H, et al. 2010. Cancer Res. 70:709.

Gene ID
929 View all products for this Gene ID
UniProt
View information about Notch 4 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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