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Purified anti-human PLAUR (uPAR) Antibody

Pricing & Availability
Clone
A20095I (See other available formats)
Regulatory Status
RUO
Other Names
CD87, MO3, Plasminogen activator urokinase receptor, uPAR, URKR
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
A20095I_PURE_PLAUR_Antibody_022024
Direct ELISA showing purified anti-human PLAUR (uPAR) (clone A20095I) bound to plate-immobilized recombinant human PLAUR (uPAR) (Cat. Nos. 559702, 559704, and 559706). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Goat anti-mouse IgG (Cat. No. 405306). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
  • A20095I_PURE_PLAUR_Antibody_022024
    Direct ELISA showing purified anti-human PLAUR (uPAR) (clone A20095I) bound to plate-immobilized recombinant human PLAUR (uPAR) (Cat. Nos. 559702, 559704, and 559706). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Goat anti-mouse IgG (Cat. No. 405306). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
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632152 100 µg £288
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Description

PLAUR (plasminogen activator urokinase receptor), also known as urokinase plasminogen activator-receptor (uPAR), is part of the plasminogen activation system, which is involved in tissue reorganization, cell migration, cell cycle regulation, and cell adhesion. PLAUR was originally identified as a saturable binding site for urokinase on the cell surface, and thus, restricting plasminogen activation to the immediate vicinity of the cell membrane. Due to lack of transmembrane or cytoplasmic domains, PLAUR may be anchored to the plasma membrane by a glycosyl-phosphatidylinositol (GPI) moiety following cleavage of the nascent polypeptide near its C-terminus. A soluble form of PLAUR has been identified. The membrane-bound PLAUR is expressed on granulocytes, monocytes/macrophages, dendritic cells, fibroblasts, endothelial cells, and keratinocytes. This molecule plays a role in cell adhesion and chemotaxis through binding to β1, β2, and β3 integrins. Recently, it was found that soluble PLAUR is increased in plasma of patients with active lupus nephritis, suggesting an association with pathological features of the disease. In addition, soluble PLAUR has been linked as a causative factor and also a biomarker of several diseases including tumor metastasis, focal and segmental glomerulosclerosis, hepatocellular carcinoma, and coronary artery disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Recombinant PLAUR (uPAR) Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

Direct ELISA - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.

Additional Product Notes

This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone A20095I) could function as a capture antibody when paired with biotin anti-human PLAUR (uPAR) (Cat. No. 631453) (clone A20095T) as the detection antibody and using Recombinant Human PLAUR (uPAR) (carrier-free) protein (Cat. Nos. 559702, 559704, and 559706) as the assay standard.

RRID
AB_3106038 (BioLegend Cat. No. 632152)

Antigen Details

Structure
Monomer
Distribution

Granulocytes, monocytes/macrophages, dendritic cells, fibroblasts, endothelial cells, keratinocytes, and activated T cells

Function
Convert plasminogen to plasmin, cell adhesion, and chemotaxis
Ligand/Receptor
uPA; LRP1 (CD91)
Cell Type
Embryonic Stem Cells
Biology Area
Angiogenesis, Cell Biology, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References
  1. Llinas P, et al. 2005. EMBO J. 24:1655-63.
  2. Beaufort N, et al. 2004. J Immunol. 172:540-9.
  3. Tarui T, et al. 2001. J Biol Chem. 276:3983-90.
  4. Qin DD, et al. 2014. Lupus. 24:546-57.
  5. Thuno M, et al. 2009. Dis Markers. 27:157-72.
  6. Alfano D, et al. 2009. Front Biosci. 14:3782-94.
  7. Spinale JM, et al. 2014. Kidney Int. 87:564-74.
  8. Chounta A, et al. 2014. Liver Int. 35:601-7.
  9. Harskamp RE and Roe MT. 2014. J Am Heart Assoc. 3:e001431.
Gene ID
5329 View all products for this Gene ID
UniProt
View information about PLAUR on UniProt.org
Go To Top Version: 1    Revision Date: 02/22/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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