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Purified anti-human Podocalyxin Antibody

Pricing & Availability
Clone
mAb 84 (See other available formats)
Regulatory Status
RUO
Other Names
GCTM-2 antigen, Gp200, Podocalyxin-like protein 1, PC, PCLP-1, PODXL, PCLP, PCLP1, GCTM2
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
mAb_84_PURE_Podocalyxin_Antibody_090117
iPS cells (NC6) were stained with purified PODXL (clone mAb 84, filled histogram) or mouse IgM, κ isotype control (open hisotgram), followed by anti-mouse IgM PE.
  • mAb_84_PURE_Podocalyxin_Antibody_090117
    iPS cells (NC6) were stained with purified PODXL (clone mAb 84, filled histogram) or mouse IgM, κ isotype control (open hisotgram), followed by anti-mouse IgM PE.
  • mAb_84_PURE_Podocalyxin_Antibody_Dot_1_081817
    Human teratocarcinoma cell line, NCCIT, stained with SSEA-4 APC and purified PODXL (clone mAb 84, left) or mouse IgM, κ isotype control (right), followed by anti-mouse IgM PE.
  • mAb_84_PURE_Podocalyxin_Antibody_Dot_2_081817
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374302 100 µg £185
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Description

Podocalyxin-like protein-1 (also known as PODXL, GP200, PCLP1 and GCTM2) is expressed on the surface of undifferentiated human ES cells and glomerular podocytes. PODXL was originally described as the main sialoprotein expressed by glomerular epithelial cells. Recently PODXL been associated with several forms of cancer and is being targeted as a possible therapeutic. Clone mAb 84 is reported to recognize and have cytotoxic affects against undifferentiated human ES cells. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Cyt, ICC, IP, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for relevant formats) include: WB, IP, cytotoxicity. The LEAF purified format is recommended for cytotoxicity assays. 

RRID
AB_2715813 (BioLegend Cat. No. 374302)

Antigen Details

Structure
Sialomucin family
Distribution

Stem cells, Glomerular podocytes

Function
Stem cell differentiation, regulatory factor
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, Immunology
Antigen References

1. Kerjaschki D, et al. 1984. J Cell Bio. 98:1591.
2. Horvat R, et al. 1986. J Cell Bio. 102:484.
3. Choo AB, et al. 2008. Stem Cells. 26:1454.
4. Tan HL, et al. 2009. Stem Cells. 27:1792.
5. Nielsen JS, et al. 2009. J Am Soc Nephrol. 20:1669.

Gene ID
5420 View all products for this Gene ID
UniProt
View information about Podocalyxin on UniProt.org
Go To Top Version: 2    Revision Date: 01/19/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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