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Purified anti-human SSEA-4 Antibody

Pricing & Availability
Clone
MC-813-70 (See other available formats)
Regulatory Status
RUO
Other Names
Stage-Specific Embryonic Antigen-4, SSEA4
Isotype
Mouse IgG3, κ
Ave. Rating
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Product Citations
publications
MC-813-70_Pure_061208.jpg
Human teratocarcinoma cell line, NCCIT, stained with purified MC-813-70, followed by anti-mouse IgG PE
  • MC-813-70_Pure_061208.jpg
    Human teratocarcinoma cell line, NCCIT, stained with purified MC-813-70, followed by anti-mouse IgG PE
  • MC-813-70_Purified_SSEA-4_Antibody_ICC_040720_updated.png
    NTERA-2 cells were fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 5 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2.5 µg/mL SSEA-4 Antibody (MC-813-70) and followed by DyLight™ 594 goat anti-mouse IgG (red) for one hour at room temperature. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
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330401 25 µg £89
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330402 100 µg £185
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Description

The MC813-70 antibody reacts with a carbohydrate antigen which is named as Stage-Specific Embryonic Antigen-4 (SSEA-4). It is a terminal structure of globo-series glycolipids (NeuAcα2-3Galβ1-3GalNAcβ1-R). SSEA-4 is expressed on human teratocarcinoma cells (EC), embryonic stem cells (ESC), embryonic germ cells (EG), and preimplantation embryos, subset of mesenchymal stem cells, erythrocytes, and erythromyeloid leukemia cell line K562. The expression of SSEA-3 on human ESC is downregulated upon differentiation. SSEA-4 is also expressed by mouse on unfertilized eggs and preimplantation to early cleaved embryos.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Mouse, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human embryonic carcinoma cell line 2102Ep
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide with stabilizer.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC - Verified
IHC-P, IHC-F, ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. For immunocytochemistry, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: ELISA1 and immunohistochemistry1 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Kannagi R, et al. 1983. The EMBO J. 2:2355
Product Citations
  1. Yang L, et al. 2023. JCI Insight. 8: . PubMed
  2. van der Graaf LM, et al. 2020. Stem Cell Research. 39:101498.. PubMed
  3. Giri S, et al. 2020. Stem Cell Research. 39:101494.. PubMed
  4. Daoutsali E et al. 2019. Stem Cell Res. 1723:19:00 . PubMed
  5. Yang L, et al. 2018. Am J Physiol Lung Cell Mol Physiol. 9:1420. PubMed
  6. Meraviglia V, et al. 2020. Stem Cell Res. 101764:45. PubMed
  7. Schmid B, et al. 2021. Stem Cell Research. 52:102240. PubMed
  8. Yang L, et al. 2021. JCI Insight. 6:. PubMed
  9. Kim KP, et al. 2020. Stem Cell Research. 72.63819444. PubMed
  10. Themeli M, et al. 2020. Stem Cell Reports. 14:300. PubMed
  11. Xia H, et al. 2021. Am J Physiol Lung Cell Mol Physiol. 320:L926. PubMed
  12. Gao X et al. 2018. Stem cell research. 31:193-196 . PubMed
  13. van der Wal E, et al. 2020. Stem Cell Research. 40:101560.. PubMed
  14. Chen X, et al. 2017. Nat Commun. 10.1038/s41467-017-00687-1. PubMed
  15. Ramovs V, et al. 2021. Stem Cell Res. 57:102582. PubMed
  16. Buijsen RAM et al. 2018. Stem cell research. 29:125-128 . PubMed
  17. Beisang DJ, et al. 2020. Sci Rep. 10:11162. PubMed
  18. Frederiksen HR et al. 2019. Stem cell research. 1725:03:00 . PubMed
  19. Bouma MJ, et al. 2019. Stem Cell Research. 41:101654. PubMed
  20. Frederiksen HR et al. 2019. Stem cell research. 1723:28:00 . PubMed
  21. Meraviglia V, et al. 2021. Stem Cell Research. 54:102426. PubMed
  22. van Helden RWJ, et al. 2021. Stem Cell Research. 53:102374. PubMed
RRID
AB_1089208 (BioLegend Cat. No. 330401)
AB_1089208 (BioLegend Cat. No. 330402)

Antigen Details

Structure
Carbohydrate epitope on glycosphingolipids
Distribution

Human teratocarcinoma cells (EC), embryonic stem cells (ESC), embryonic germ cells (EG), preimplantation embryos, and erythrocytes; mouse oocytes, early cleaved embryos

Function
Regulation of differentiation
Cell Type
Embryonic Stem Cells, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Antigen References

1. Kannagi R, et al. 1983. The EMBO J. 2:2355
2. Henderson JK, et al. 2002. Stem Cells. 20:329
3. Brimble SN, et al. 2007. Stem Cells. 25:54
4. Battula VL, et al. 2007. Differentiation. 75:279

Gene ID
NA
UniProt
View information about SSEA-4 on UniProt.org
Go To Top Version: 6    Revision Date: 12/04/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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