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- Clone
- 8e11 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Stage-Specific Embryonic Antigen-5
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human teratocarcinoma cell line, NCCIT, was stained with purified SSEA-5 (clone 8e11, filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 355201 | 50 µg | £85 | ||||
| 355202 | 200 µg | £221 | ||||
Stage-Specific Embryonic Antigen-5 (SSEA-5) is an oligosaccharide that contains the motif Fuc1-2Galβ1-3GlcNAcβ, which is characteristic of H-1 antigens. SSEA-5 is expressed on undifferentiated embryonic stem cells and pluripotent stem cells. It is developmentally regulated, and its expression is lost with cell differentiation.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human embryonic stem cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1 and immunoprecipitation1.
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Application References
(PubMed link indicates BioLegend citation) -
- Tang C, et al. 2011. Nat Biotechnol. 29:829. (IHC, IP)
- Product Citations
-
- RRID
-
AB_2561465 (BioLegend Cat. No. 355201)
AB_2561465 (BioLegend Cat. No. 355202)
Antigen Details
- Structure
- Glycan, H type-1 backbone, 127, and >190 kD
- Distribution
-
Embryonic stem cells, pluripotent stem cells
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Immunology, Stem Cells
- Antigen References
-
1. Andrews PW. 2011. Nat. Biotechnol. 29:803.
2. Itskovitz-Eldor J. 2011. Cell. Stem. Cell. 9:291. - Gene ID
- NA
- UniProt
- View information about SSEA-5 on UniProt.org
Related FAQs
Other Formats
View All SSEA-5 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human SSEA-5 | 8e11 | FC,IHC-F,IP |
| PE anti-human SSEA-5 | 8e11 | FC |
| FITC anti-human SSEA-5 | 8e11 | FC |
| APC anti-human SSEA-5 | 8e11 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human SSEA-5
Human teratocarcinoma cell line, NCCIT, was stained with pur... -
PE anti-human SSEA-5
Human teratocarcinoma cell line, NCCIT, was stained with SSE... -
FITC anti-human SSEA-5
Human teratocarcinoma cell line NCCIT, was stained with SSEA... -
APC anti-human SSEA-5
Human teratocarcinoma cell line, NCCIT, was stained with SSE...
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