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Purified anti-human TRA-1-81 Antibody

Pricing & Availability
Clone
TRA-1-81 (See other available formats)
Regulatory Status
RUO
Other Names
Podocalyxin
Isotype
Mouse IgM, κ
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Product Citations
publications
a-Tra-1-81_Pure_061208.jpg
Human teratocarcinoma cell line, NCCIT, stained with purified TRA-1-81, followed by anti-mouse IgM PE
  • a-Tra-1-81_Pure_061208.jpg
    Human teratocarcinoma cell line, NCCIT, stained with purified TRA-1-81, followed by anti-mouse IgM PE
  • b-TRA-1-81_Purified_WB_052314.jpg
    Western blot analysis of NTERA-2 (lane 1), NF-1 (lane 2) and Hela (lane 3) cells using anti-TRA-1-81 antibody (TRA-1-81). GAPDH (poly6314) was used a loading control.
  • c-TRA-1-81_PURE_TRA-1-81_ICC_012121
    HUES-9 ES cell colony was stained with Tra-1-81 antibody at 1:400 and then secondarily stained with Alexa Fluor® 546 goat anti-mouse IgM at 1:250 (red). Nuclei were counterstained with DAPI (blue). Image was provided by Eric O'Connor and Karl Marquez, Sanford Consortium for Regenerative Medicine.
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330702 100 µg £188
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Description

The TRA-1-81 antibody reacts with a 200-240 kD cell surface antigen expressed by human embryonic stem cells (ESC), embryonic germ cells (EG), and embryonal carcinoma cells. The expression of TRA-1-81 on human ESC is downregulated upon differentiation. Further studies have shown that TRA-1-81 is a neuroaminidase-resistant carbohydrate epitope expressed on podocalyxin, a member of mucin-like glycoprotein with CD34 family. Podocalyxin is a type I tranmembrane sialoprotein with anti-adhesion property.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human embryonic carcinoma cell line 2102Ep cl.2A6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
WB - Verified
IP, ICC, IHC, ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence cell staining1, immunoprecipitation1, Western blotting1,2, ELISA1, and immunohistochemistry1 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Andrews PW, et al. 1984. Hybridoma. 3:347.
  2. Schopperle WM and Dewolf WC. 2007. Stem Cells. 25:723.
Product Citations
  1. Claassen JN et al. 2019. Stem Cell Res. 1723:12:00 . PubMed
  2. Devine MJ, et al. 2011. Nat Commun. 1.263888889. PubMed
  3. Hey CAB et al. 2018. Stem Cell Res. 33:46-50 . PubMed
  4. Themeli M, et al. 2020. Stem Cell Reports. 14:300. PubMed
  5. Sanchez–Duffhues G, et al. 2020. Stem Cell Research. 72.29097222. PubMed
  6. Chen X, et al. 2017. Nat Commun. 10.1038/s41467-017-00687-1. PubMed
  7. Hey CAB et al. 2018. Stem cell research. 31:235-239 . PubMed
  8. Frederiksen HR et al. 2019. Stem cell research. 1725:03:00 . PubMed
  9. Kamata M, et al. 2010. PLoS One. 5:e11834. PubMed
  10. Frederiksen HR et al. 2019. Stem cell research. 1723:28:00 . PubMed
  11. Schmid B et al. 2019. Stem Cell Res. 1723:09:00 . PubMed
  12. McLenachan S et al. 2019. Stem Cell Res. 1723:17:00 . PubMed
RRID
AB_1089240 (BioLegend Cat. No. 330702)

Antigen Details

Structure
Mucin-like protein, CD34 family member, 200-240 kD
Distribution

embryonic stem cells (ESC), embryonic germ cells (EG), embryonal carcinoma cells

Function
Anti-adhesion
Cell Type
Embryonic Stem Cells
Biology Area
Cell Biology, Immunology, Stem Cells
Antigen References

1. Andrews PW, et al. 1984. Hybridoma. 3:347.
2. Schopperle WM and Dewolf WC. 2007. Stem Cells. 25:723.
3. Takeda T, et al. 2000. Mol. Biol. Cell. 11:3219.

Gene ID
5420 View all products for this Gene ID
UniProt
View information about TRA-1-81 on UniProt.org
Go To Top Version: 4    Revision Date: 01/21/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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