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Purified anti-IDO1 Antibody

Pricing & Availability
Clone
2E2/IDO1 (See other available formats)
Regulatory Status
RUO
Other Names
Indolamine 2,3-dioxygenase, Indole 2,3-dioxygenase, Indoleamine-pyrrole 2,3-dioxygenase
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
2E2slashIDO1_PURE_IDO1_Antibody_WB_082616
Total cell lysates (15 µg protein) from 293E untransfected or transfected with mouse IDO1 were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with purified anti-IDO1 antibody (clone 2E2/IDO1). Proteins were visualized using a goat anti-mouse IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control.
  • 2E2slashIDO1_PURE_IDO1_Antibody_WB_082616
    Total cell lysates (15 µg protein) from 293E untransfected or transfected with mouse IDO1 were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with purified anti-IDO1 antibody (clone 2E2/IDO1). Proteins were visualized using a goat anti-mouse IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control.
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654001 25 µg £94
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654002 100 µg £189
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Description

IDO1 is also known as Indolamine 2,3-dioxygenase, Indole 2,3-dioxygenase, and Indoleamine-pyrrole 2,3-dioxygenase. IDO is a ubiquitously expressed cytoplasmic protein with a predicted molecular weight of approximately 45 kD. Indoleamine 2,3-dioxygenase (IDO) is one the best known IFN-γ inducible genes. The product of IDO gene catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine. IDO has been implicated to protect against intracellular and extracellular pathogens. It also has been shown to maintain the special immune suppressive status of immune-privileged sites such as the brain, eyes, kidney, and placenta.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant mouse IDO1.
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Protein G affinity purified.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 2E2/IDO1 reacts with mouse IDO1. It does not appear to react with mouse IDO2.

Clone 2E2/IDO1 is the same clone as anti-IDO1 clone 2E2.6.

Product Citations
  1. Ji R, et al. 2021. PLoS One. 16:e0258204. PubMed
  2. Yuk H, et al. 2021. Antioxidants (Basel). 10:. PubMed
  3. Moritsch S, et al. 2022. Oncoimmunology. 11:2127271. PubMed
RRID
AB_2561779 (BioLegend Cat. No. 654001)
AB_2561779 (BioLegend Cat. No. 654002)

Antigen Details

Structure
403 amino acids with a predicted molecular weight of 45 kD.
Distribution

Cytoplasm

Function
Indoleamine 2,3-dioxygenase (IDO) is an IFN-γ inducible gene. It catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine. IDO has been implicated to protect against intracellular and extracellular pathogens.
Interaction
This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin.
Biology Area
Cell Biology, Immunology
Antigen References

1. Habara-Ohkubo A, et al. 1991. Gene 105:221.
2. Munn DH, et al. 2002. Science 297:1867.
3. Frumento G, et al. 2002. J. Exp. Med. 196:459.
4. Muller AJ, et al. 2005. Nature Med. 11:312.
5. Lob S, et al. 2009. Nature Rev. Cancer 9:445.

Gene ID
15930 View all products for this Gene ID
UniProt
View information about IDO1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 10/19/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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