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Purified anti-Iduna (RNF146) Antibody

Pricing & Availability
Clone
N201/35 (See other available formats)
Regulatory Status
RUO
Other Names
RING-type E3 Ubiquitin Transferase RNF146, Iduna. Ring Finger Protein 146
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
N201slash35_PURE_Iduna_RNF146_Antibody_102021
Whole tissue membrane cell extracts (15 µg total protein) from human and rat brain were heated at 37°C for 30 minutes, resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL of purified anti-Iduna (RNF146) antibody (clone N201/35) overnight at 4 °C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular weight marker.
  • N201slash35_PURE_Iduna_RNF146_Antibody_102021
    Whole tissue membrane cell extracts (15 µg total protein) from human and rat brain were heated at 37°C for 30 minutes, resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL of purified anti-Iduna (RNF146) antibody (clone N201/35) overnight at 4 °C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular weight marker.
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602052 100 µg £113
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Description

Ring finger protein 146 (RNF146), also known as Iduna, is a poly-ADP-ribose (PAR)-dependent E3 ubiquitin-protein ligase that promotes the degradation of PARsylated proteins. The ability of RNF146 to bind to PAR and thus inhibit PAR dependent cell death, or parthanatos, contributes to its role in DNA repair and cell survival and overall neuroprotective qualities. RNF146 has been identified as an NMDA receptor-induced survival gene that protects against NMDA receptor mediated excitotoxicity and ischemia-induced neuronal injury. RNF146 is a positive regulator of the Wnt/ β-catenin signaling pathway through the ubiquitylation of AXIN following its degradation by tankyrase and has been implicated in embryonic and postnatal osteoblast development, bone remodeling, and energy metabolism via coordination of β-catenin signaling and Hippo pathways within osteoblasts.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length mouse Iduna (RNF146) protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone has been tested for IHC and failed.

RRID
AB_2904447 (BioLegend Cat. No. 602052)

Antigen Details

Structure
RNF146 is a 359 amino acid protein with a predicted molecular weight of 39 kD.
Distribution

Ubiquitously expressed /Nucleus and Cytosol

Function
Wnt signaling pathway, Ubiquitin conjugation pathway
Antigen References
  1. Andrabi S, et al. 2011. Nat. Med. 17:692.
  2. Kang H, et al. 2011. PNAS. 108:14103.
  3. Matsumoto  Y, et al. 2017. J. Clin. Invest. 127:2612.
  4. Zhang Y, et al. 2011. Nat. Cell Biol. 13:623.
Gene ID
81847 View all products for this Gene ID
UniProt
View information about Iduna on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/20/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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