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Purified anti-IGBP-1 Antibody (Previously Covance catalog# MMS-5088)

Pricing & Availability
Clone
5F6 (See other available formats)
Regulatory Status
RUO
Other Names
Alpha4, Immunoglobulin-binding protein 1, CD79a-binding protein 1, phosphoprotein, component, 52 kD, lymphocyte signal transduction molecule alpha 4, ALPHA-4, IBP1, renal carcinoma antigen NY-REN-16
Previously
Covance Catalog# MMS-5088
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
5F6_PURE_IGBP1_WB_123118
Western blot of purified anti-IGBP-1 antibody (clone 5F6). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate: Lane 5: 20 µg of HeLa cell lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 5F6_PURE_IGBP1_WB_123118
    Western blot of purified anti-IGBP-1 antibody (clone 5F6). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate: Lane 5: 20 µg of HeLa cell lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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828601 100 µL £189
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Description

IGBP1 (also known as Alpha4) , binds to PP2A C in the absense of A and a B subunit in a catalytically inactive state to prevent C proteosomal degradation.  If alpha4 levels are low, PP2A levels can decrease, leading to increased tau protein phosphorylation and aggregation. Protein phosphatase 2A is a heterotrimeric protein serine/threonine phosphatase consisting of a 36-kD catalytic C subunit (alpha and beta isoforms), a 65-kD structural A subunit (alpha and beta isoforms), and a variable regulatory B subunit. When the PP2A catalytic C subunit associates with the A and B subunits several species of holoenzymes are produced with distinct functions and characteristics. Ubiquitously expressed, PP2A is involved in a broad range of cellular processes, including membrane receptor desensitization, signal transduction, intermediary metabolism, transcriptional regulation and control of DNA replication, and mitosis. The PP2A phosphatase family accounts for the majority of Ser/Thr phosphatase activity in the brain and specifically regulates tau phosphorylation, both directly as tau phosphatase and indirectly by regulating the activities of several tau kinases which include CaM Kinase II, PKA, MAP kinase kinase (MEK 1/2), extracellular regulated kinase (ERK 1/2), GSK-3β, and P70S6 kinase, thus regulating tau microtubule binding in the brain.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against an N-terminal 6-HIS tagged fusion protein corresponding to full-length mouse Alpha4 protein.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
ICC, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Kong M, et al. 2009. Mol Cell. 36:51. (IP, ICC, WB)
RRID
AB_2564922 (BioLegend Cat. No. 828601)

Antigen Details

Structure
Expected MW: 40 kD
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Phospho-Proteins
Gene ID
8518 View all products for this Gene ID
UniProt
View information about IGBP-1 on UniProt.org
Go To Top Version: 4    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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