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Purified anti-IκB-α Antibody

Pricing & Availability
Clone
3D6C02 (See other available formats)
Regulatory Status
RUO
Other Names
Nuclear factor of kappa light chain enhancer in B cells-inhibitor alpha, IKBA
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
1_3D6C02_IkB-a_Purified_Antibody_1_WB_091714.jpg
Western blot analysis of cell lysates from HeLa (Human), Raw264.7 (Mouse) and UMR106 (Rat) using IκB-α Mouse primary antibody and HRP Goat anti-Mouse secondary antibody (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control.
  • 1_3D6C02_IkB-a_Purified_Antibody_1_WB_091714.jpg
    Western blot analysis of cell lysates from HeLa (Human), Raw264.7 (Mouse) and UMR106 (Rat) using IκB-α Mouse primary antibody and HRP Goat anti-Mouse secondary antibody (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control.
  • 2_3D6C02_IkB-a_Purified_Antibody_2_WB_122017
    Total lysates (15 µg protein) from HepG2 (Human) and CHO (Hamster) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:5000 (0.1 µg/ml) purified anti-IκB-α antibody, clone 3D6C02. Proteins were visualized using chemiluminescence detection by incubation with HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • 3_3D6C02_IkB-a_Purified_Antibody_1_IF_082917
    Hela cells were fixed with cold methanol at -20°C for 15 minutes and blocked with 5% FBS for 60 minutes. The cells were then intracellularly stained with (A) 2 µg/ml anti-mouse IgG2b, κ or (B) 2 µg/ml IκB-α antibody (clone 3D6C02), (C) 0.5 µg/ml IκB-α antibody (clone 3D6C02), or (D) 0.2 µg/ml IκB-α antibody (clone 3D6C02), and incubated overnight at 4°C followed by 2µg/ml (1:250) Alexa Fluor® 594 (Red) goat anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI (Blue). The image was captured with a 60X objective. Exposure times: (A) 1/30, (B) 1/50, (C) 1/50, (D) 1/50.
  • 4_3D6C02_IkB-a_Purified_Antibody_2_IF_091714.jpg
    HeLa cells were stained with purified anti-IκBα (clone 3D6C02) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG (green) antibody and Alexa Fluor® 594 conjugated phalloidin (red). Nuclei were stained with DAPI (blue).
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662402 100 µg £157
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Description

IκB-α is an inhibitory protein that binds to the NF-κB complex and retains this complex in the cytoplasm where it cannot activate transcription. Upon cellular stimulation, the IκB proteins are phosphorylated and degraded via the ubiquitin pathway allowing for nuclear translocation of NF-κB. The NF-κB complex can then bind DNA to activate gene expression of multiple proteins involved in inflammation, cellular proliferation, differentiation, and apoptosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, Hamster
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length human IκB-α recombinant protein expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg/ml (1:1000 - 1:5000 dilution). For immunocytochemistry, the suggested use is 0.5 - 1.0 µg/ml (1:500-1:1000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody weakly reacts with mouse and rat. Based on in-house testing, clone 3D6C02 does not block clone 25/IkBa/MAD-3, and only partially blocks L35A5 also raised against I?B-a.

Product Citations
  1. Buono L, et al. 2023. J Clin Med. 12:. PubMed
  2. Smaldone G, et al. 2021. Sci Rep. 11:18237. PubMed
  3. Millen S, et al. 2020. Retrovirology. 17:30. PubMed
RRID
AB_2564258 (BioLegend Cat. No. 662402)

Antigen Details

Structure
Inhibitory protein of the NF-κB complex that contains multiple ankyrin repeats.
Distribution

Cytoplasm

Function
Regulates transcriptional activity of NF-κB by binding the complex and trapping it in the cytoplasm. Serine phosphorylation of IκB targets it for destruction via the ubiquitination pathway, which allows the NF-κB complex to translocate to the nucleus.
Interaction
NFkB1, SUMO4, p53, ribosomal S6 kinase 1
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Signal Transduction
Molecular Family
Tumor Suppressors
Antigen References

1. Baeuerle PA. 1998. Cell 95:729.
2. Hoffmann A, et al. 2002. Science 298:1241.
3. Ito CY, et al. 1995. Genomics 29:490.

Gene ID
4792 View all products for this Gene ID
UniProt
View information about IkappaB-alpha on UniProt.org
Go To Top Version: 2    Revision Date: 09/19/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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