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Purified anti-IκB-α Antibody

Pricing & Availability
Clone
W16166B (See other available formats)
Regulatory Status
RUO
Other Names
Nuclear factor of kappa light chain enhancer in B cells-inhibitor alpha, IKBA, IκBα
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W16166B_PURE_IkappaBalpha_Antibody_1_06142018
Total cell lysate (15 µg protein) from A20, EL-4, NIH3T3, HeLa and Jurkat cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose membrane, and probed with 1:500 (1 µg/mL) diluted purified anti-IκB-α antibody (clone W16166B, upper). Proteins were visualized using 1:3000 diluted HRP goat anti-rat-IgG secondary antibody (Cat. No. 405405) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (1:2000 diluted, clone 2F1-1) was used as a loading control (lower). Lane M is the MW ladder.
  • W16166B_PURE_IkappaBalpha_Antibody_1_06142018
    Total cell lysate (15 µg protein) from A20, EL-4, NIH3T3, HeLa and Jurkat cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose membrane, and probed with 1:500 (1 µg/mL) diluted purified anti-IκB-α antibody (clone W16166B, upper). Proteins were visualized using 1:3000 diluted HRP goat anti-rat-IgG secondary antibody (Cat. No. 405405) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (1:2000 diluted, clone 2F1-1) was used as a loading control (lower). Lane M is the MW ladder.
  • W16166B_PURE_IkappaBalpha_Antibody_2_06142018
    Total cell lysate (300 µg protein) from NIH3T3 were incubated with 2.5 µg mouse IgG1 isotype control (lane 1) or anti-IκB-α antibody (lane 2) at 4°C for 3 hours, followed by incubating the Protein-G beads overnight. Immunoprecipitates and total protein (10% of input, lane 3) were eluted with SDS loading buffer and resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 1:500 (1 µg/mL) diluted purified anti- IκB-α antibody (clone W16166B, upper). Proteins were visualized using 1:3000 diluted HRP goat anti-rat IgG secondary antibody (Cat. No. 405405) and chemiluminescence detection. Lane M is the MW ladder. IgGHC: IgG heavy chain, IgGHC: IgG light chain.
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609101 25 µg £70
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609102 100 µg £174
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Description

IκB-α is an inhibitory protein that binds to the NF-κB complex and retains this complex in the cytoplasm where it cannot activate transcription. Upon cellular stimulation, the IκB proteins are phosphorylated and degraded via the ubiquitin pathway allowing for nuclear translocation of NF-κB. The NF-κB complex can then bind DNA to activate gene expression of multiple proteins involved in inflammation, cellular proliferation, differentiation, and apoptosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse IkB-α recombinant protein (full length) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.125-1.0 µg per ml (dilution of 1:500-1:4000). For immunoprecipitation, the suggested use of this reagent is 2.5 - 3.00 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Weakly cross reacts with human I?Ba.

This clone can not be used for ICC application.

RRID
AB_2734508 (BioLegend Cat. No. 609101)
AB_2734508 (BioLegend Cat. No. 609102)

Antigen Details

Structure
314 amino acids, with predicted molecular weight 35.1 kD. Inhibitory protein of the NF-κB complex that contains multiple ankyrin repeats.
Distribution

Cytoplasm

Function
Regulates transcriptional activity of NF-κB by binding the complex and trapping it in the cytoplasm. Serine phosphorylation of IκB targets it for destruction via the ubiquitination pathway, which allows the NF-κB complex to translocate to the nucleus.
Interaction
Interacts with NFκB1, SUMO4, p53, ribosomal S6 kinase 1.
Biology Area
Cell Biology, Signal Transduction
Antigen References
  1. Baeuerle PA. 1998. Cell. 95:729.
  2. Hoffmann A, et al. 2002. Science. 298:1241.
  3. Ito CY, et al. 1995. Genomics. 29:490.
Gene ID
18035 View all products for this Gene ID
UniProt
View information about IkappaB-alpha on UniProt.org
Go To Top Version: 1    Revision Date: 06/21/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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