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Purified anti-IKBKB (IKKβ) Antibody

Pricing & Availability
Clone
W15160A (See other available formats)
Regulatory Status
RUO
Other Names
Inhibitor of nuclear factor kappa-B kinase subunit beta, IKK“Ä’, IKKbeta, NFKBIKB, IKKB, IKK2
Isotype
Mouse IgG1, κ
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Product Citations
publications
W15160A_PURE_IKBKB_Antibody_WB_110617
Total lysates (10 µg protein) from HeLa and HeLa IKBKB CRISPR/Cas9 knockout (KO) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1 µg/ml) purified anti-IKBKB (IKKβ) antibody, clone W15160A (upper). Proteins were visualized using chemiluminescence detection by incubation with HRP goat anti-mouse-IgG secondary antibody (cat#405306). Direct-Blot™ HRP anti-β-actin Antibody (Cat#643807) was used as a loading control (lower). Lane M is the Molecular weight ladder. The band observed above 50 Kd in the HeLa IKBKB KO cells is IKBKB as CRISPR/Cas9 technology can cause exon skipping, which in turn results in expression of partial protein.
  • W15160A_PURE_IKBKB_Antibody_WB_110617
    Total lysates (10 µg protein) from HeLa and HeLa IKBKB CRISPR/Cas9 knockout (KO) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1 µg/ml) purified anti-IKBKB (IKKβ) antibody, clone W15160A (upper). Proteins were visualized using chemiluminescence detection by incubation with HRP goat anti-mouse-IgG secondary antibody (cat#405306). Direct-Blot™ HRP anti-β-actin Antibody (Cat#643807) was used as a loading control (lower). Lane M is the Molecular weight ladder. The band observed above 50 Kd in the HeLa IKBKB KO cells is IKBKB as CRISPR/Cas9 technology can cause exon skipping, which in turn results in expression of partial protein.
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688402 100 µg £185
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Description

NF-kappa-B (NF-κB) signaling pathway is involved in many biological responses such as immune response, growth control, DNA damage and other cellular stresses. This pathway is regulated by the inhibitory IKK proteins (composed by IKKα, IKBKB/IKKβ and IKKγ) which complex with NF-κB/Rel transcription factors in the cytosol. As part of the IKK complex, IKBKB phosphorylates inhibitors of NF-κB on 2 serine residues. These phosphorylations cause polyubiquitination and subsequent degradation of the NF-κB inhibitors. In turn, free NK-κB translocates into nucleus and activates the downstream gene transcription. Upon cytokine stimulation, phosphorylation of Ser-177 and -181 enhances IKBKB activity; on the other hand, acetylation of Thr-180 residue prevents IKKβ phosphorylation and activation, thus blocking the inhibition of NF-κB pathway.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human IKBKB recombinant protein (520-745 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2617026 (BioLegend Cat. No. 688402)

Antigen Details

Structure
756 amino acids with a predicted molecular weight of 86.6 kD. Contains a N-terminal kinase domain and a leucine zipper region responsible for dimerization.
Distribution

Nucleus and cytoplasm.

Function
IKBKB is a serine kinase that participates in the NF-κB signaling pathway. It is activated by a variety of stimuli, including inflammatory cytokines, pathogen infections, and other cellular stresses.
Interaction
Component of the IKK core complex. Interacts with NALP2, TICAM1, FAF1, ATM, NIBP, ARRB1, ARRB2, TRIM21, NLRC5, PDPK1, EIF2AK2/PKR, ZNF268, IKBKE, NAA10, and FOXO3.
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Sasaki Y, et al. 2016. Curr. Top. Microbiol. Immunol. 393:177.
2. Nielsen C, et al. 2014. J. Clin. Immunol. 34:916.
3. Amaya M, et al. 2014. Pathog. Dis. 72:32.
4. Hinz M, et al. 2014. EMBO Rep. 15:46.
5. Mousallem T, et al. 2014. Blood. 124:2046.
6. Schmid JA, et al. 2008. Cytokine Growth Factor Rev. 19:157.

Gene ID
3551 View all products for this Gene ID
UniProt
View information about IKBKB on UniProt.org
Go To Top Version: 2    Revision Date: 01/13/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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