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Purified anti-IL-1α Antibody

Pricing & Availability
Clone
A15032A (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin 1, alpha, IL-1A, IL1F1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A15032A_PURE_IL1alpha_Antibody_1_WB_042916
Western blot analysis of 50 ng recombinant human IL-1α (lane 1), recombinant mouse IL-1α (lane 2), THP-1 untreated (lane 3), THP-1 treated with PMA for three days, then LPS for one day (lane 4), and Raw264.7 untreated (lane 5) and Raw264.7 starved overnight and treated with LPS for one day (lane 6). Anti-IL-1α (clone A15032A) antibody was used, followed by HRP anti-mouse IgG secondary antibody. Purified anti-GAPDH antibody was used as a loading control.
  • A15032A_PURE_IL1alpha_Antibody_1_WB_042916
    Western blot analysis of 50 ng recombinant human IL-1α (lane 1), recombinant mouse IL-1α (lane 2), THP-1 untreated (lane 3), THP-1 treated with PMA for three days, then LPS for one day (lane 4), and Raw264.7 untreated (lane 5) and Raw264.7 starved overnight and treated with LPS for one day (lane 6). Anti-IL-1α (clone A15032A) antibody was used, followed by HRP anti-mouse IgG secondary antibody. Purified anti-GAPDH antibody was used as a loading control.
  • A15032A_PURE_IL1alpha_Antibody_2_IF_042916
    U87-MG cells were fixed with 4% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for five minutes and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2 µg/mL anti-IL-1α (clone A15032A) antibody for two hours followed by 2 µg/mL Alexa Fluor® 594 conjugated goat anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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686502 100 µg £213
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Description

IL-1 (Interleukin-1) is a central mediator of innate immunity and inflammation. The IL-1 family includes seven ligands with agonist activity (IL-1α and IL-1β, IL-18, IL-33, IL-36α, IL-36β, IL-36γ), three receptor antagonists (IL-1RA, IL-36RA, IL-38), and an anti-inflammatory cytokine (IL-37). A tight regulation through receptor antagonists, decoy receptors, and signaling inhibitors ensures a balance between amplification of innate immunity and uncontrolled inflammation. The cytokine IL-1α is a key molecule in the IL-1 family that induces autonomous proliferation in vascular smooth muscle cells, T cell activation during antigen presentation, up-regulation of monocyte/macrophage-mediated tumor cytotoxicity, stimulation of osteoclast formation, and production of proteinases in human chondrocytes. Furthermore, IL-1α is also an early and important mediator of inflammation after injury.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human IL-1α, amino acids Ser113-Ala271 (Accession# NM_000575) was expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone A15032A weakly reacts with mouse IL-1a.

RRID
AB_2616884 (BioLegend Cat. No. 686502)

Antigen Details

Structure
271 amino acids with the predicted molecular weight of approximately 31kD.
Distribution

IL-1α is produced by monocytes, macrophages, keratinocytes, stimulated B lymphocytes, fibroblasts, endothelial cells, and epithelial cells.

Function
The cytokine IL-1α is a key molecule both in the innate and in the adaptive immune response. It induces autonomous proliferation in vascular smooth muscle cells, T cell activation during antigen presentation, up-regulation of monocyte/macrophage-mediated tumor cytotoxicity, stimulation of osteoclast formation, and production of proteinases in human chondrocytes.
Interaction
Stimulates thymocyte and B-cell proliferation.
Ligand/Receptor
Type I and II IL-1 receptors (IL-1RA and IL-1RB).
Cell Type
B cells, Neutrophils
Biology Area
Angiogenesis, Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Ledford H. 2007. Nature 450:600.
2. Boraschi D, Tagliabue, A. 2006. Vitam. Horm. 74:229.
3. Shao J, Sheng H. 2007. J. Immunol. 178:4097.
4. Elaraj DM, et al. 2006. Clin. Cancer Res. 12:1088.
5. Schett G, et al. 2016. Nat. Rev. Rheumatol. 12:14.
6. Brough D, et al. 2015. IUBMB Life 67:323.
7. Dinarello CA, et al. 2014. Mol. Med. 20 Suppl. 1:S43-58.
8. Voronov E, et al. 2014. Front Physiol. 5:114.
9. Garlanda C, et al. 2013. Immunity 39:1003.
10. Rider P, et al. 2013. Semin. Immunol. 25:430.

Gene ID
3552 View all products for this Gene ID
UniProt
View information about IL-1alpha on UniProt.org
Go To Top Version: 1    Revision Date: 05/02/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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