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- Clone
- J053B5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Epstein-Barr Virus Induced 3, EBV-Induced Gene 3 Protein, IL-27 Subunit Beta, IL-27B, IL-35 subunit
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Western blot analysis of 50 ng of recombinant human IL-27 (lane 1), 50 ng of recombinant mouse IL-27 (lane 2), and 50 ng of recombinant human IL-35 (lane 3) using anti-EBI3 antibody (clone J053B5).
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 662602 | 100 µg | £182 | ||||
EBI3 is an IL-12 p40-related protein that can heterodimerized with IL-12 p35 to form IL-35, or with an IL-12 p35-related protein, p28, to form IL-27. It is produced by antigen-presenting cells such as B cells, monocytes, and dendritic cells (DCs) following stimulation through TLR. It can regulate T cell differentiation and proliferation. Both IL-27 and IL-35 are anti-inflammatory cytokines that are able to inhibit Th17 generation, promote Th1 differentiation, increase cytolytic activity of CD8+ T cells, and impact B cell differentiation and immunoglobulin production. IL-35 is also involved in tumorigenesis and is a promising biomarker for assessing tumor progression and prognosis in clinical settings.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse EBI3
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2564303 (BioLegend Cat. No. 662602)
Antigen Details
- Structure
- 229 amino acids, predicted molecular weight of approximately 25 kD.
- Distribution
-
B cells, monocytes, and dendritic cells.
- Function
- Pro- and anti-inflammatory.
- Interaction
- IL-27 p28 subunit and IL-35 p35 subunit.
- Ligand/Receptor
- WSX-1(TCCR) and gp130.
- Cell Type
- B cells, Dendritic cells, Monocytes, Tregs
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Signal Transduction
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Pflanz S, et al. 2002. Immunity 16:779.
2. Takeda A, et al. 2003. J. Immunol. 170:4886.
3. Pflanz S, et al. 2004. J. Immunol. 172:2225.
4. Li MF, et al. 2013. Dev. Comp. Immunol. 41:514.
5. Zeng JC, et al. 2013. Int. J. Clin. Exp. Pathol. 6:1806.
6. Ouyang H, et al. 2014. Am. J. Med. Sci. 348:156. - Gene ID
- 10148 View all products for this Gene ID
- UniProt
- View information about IL-27 IL-35 on UniProt.org
Related FAQs
Other Formats
View All IL-27/IL-35 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-IL-27/IL-35 EBI3 (monomer, dimer, heterodimer) | J053B5 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-IL-27/IL-35 EBI3 (monomer, dimer, heterodimer)
Western blot analysis of 50 ng of recombinant human IL-27 (l...
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