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Purified anti-IRF3 Antibody

Pricing & Availability
Clone
12A4A35 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon regulatory factor-3 (IRF-3)
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
1_12A4A35_Purified_IRF3_Antibody_WB_062013.jpg
Total lysates (15 µg protein) from Daudi (lane 1) and A20 (lane 2) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) purified anti-IRF3 antibody clone 12A4A35 (upper). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306) or HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401) for GAPDH and chemiluminescence detection. GAPDH (poly6314, Cat. No. 631401) antibody was used as a loading control (lower). Lane M: MW ladder.
  • 1_12A4A35_Purified_IRF3_Antibody_WB_062013.jpg
    Total lysates (15 µg protein) from Daudi (lane 1) and A20 (lane 2) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) purified anti-IRF3 antibody clone 12A4A35 (upper). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306) or HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401) for GAPDH and chemiluminescence detection. GAPDH (poly6314, Cat. No. 631401) antibody was used as a loading control (lower). Lane M: MW ladder.
  • 2_12A4A35_GoChIP_IRF3_Antibody_120717.png
    Chromatin Immunoprecipitation (ChIP) was performed using Go-ChIP-Grade™ Protein G Enzymatic Kit by loading 3 µg of cross-linked chromatin samples from THP-1 cells treated with LPS with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-IRF3 (Clone 12A4A35), or B) equal amount of Purified Mouse IgG2b, κ Isotype Control Antibody. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human IFNB gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
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655701 25 µg £85
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655702 100 µg £182
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Description

IRF-3 is a member of the interferon regulatory transcription factor (IRF) family. It mediates interferon-stimulated response element (ISRE) promoter activation and functions as a molecular switch for antiviral activity. DsRNA generated during the course of a viral infection leads to IRF-3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB protein binding to dsRNA-activated factor 1, a complex which activates the transcription of genes under control of ISRE. The phosphorylated IRF-3 is under negative regulation by ubiquitin-mediated degradation. Research indicates that it plays a critical role in stimulated TLR-mediated IL-27 p28 gene expression.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial IRF-3 recombinant protein (200-427 a.a.)
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Affinity purified
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
ChIP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For ChIP applications, the suggested dilution is 1:50-1:100 by volume. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Ta A, et al. 2023. Nat Commun. 14:2035. PubMed
  2. He X, et al. 2017. PLoS One. 13(6):e1006347. PubMed
  3. Kanno T, et al. 2022. Front Immunol. 13:904875. PubMed
  4. Raychowdhury R, et al. 2021. Immunohorizons. 5:818. PubMed
RRID
AB_2562202 (BioLegend Cat. No. 655701)
AB_2562202 (BioLegend Cat. No. 655702)

Antigen Details

Structure
Human IRF-3 has 427 amino acids with predicted Mw 47.2kD and observed Mw 52kD. Contains an IRF tryptophan pentad repeat responsible for DNA binding. Mouse Irf-3 has 419 amino acids with predicted MW 46.9kD and observed Mw 45kD.
Distribution

Inactive IRF-3 continuously transits between the cytoplasm and nucleus. Serine/threonine phosphorylation or IRF-3 results in the structural changes which allow IRF-3 activation. The activated IRF3 translocates to the nucleus, where it forms a complex with CREB binding protein and activates the transcription of interferons, interferon stimulated genes, and other antiviral response genes.

Function
IRF-3 is a constitutively expressed, latent transcription factor that plays a pivotal role in type I IFN response.
Interaction
CREB binding protein, IRF-7, TICAM-1, TICAM-2, non-canonical IKB kinases TBK1 and IKKε.
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Lin R, et al. 1999. Mol. Cell Biol. 19:959.
2. Hiscott J, et al. 2007. J. Biol. Chem. 282:15325.
3. Molle C, et al. 2010. J. Immunol. 184:1784.

Gene ID
3661 View all products for this Gene ID
UniProt
View information about IRF3 on UniProt.org
Go To Top Version: 5    Revision Date: 08/24/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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