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Purified anti-IRF6 Antibody

Pricing & Availability
Clone
14B2C16 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon regulatory factor 6, IRF-6
Isotype
Mouse IgG2a, κ
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Product Citations
publications
14B2C16_PURE_IRF6_Antibody_1_WB_071816
Total lysates (15 µg protein) from Jurkat (lane 1), HaCaT (lane 2) and NIH3T3 (lane 3) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) purified anti-IRF6 antibody (upper). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306) or HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401) for GAPDH and chemiluminescence detection. GAPDH (poly6314, Cat. No. 631401) antibody was used as a loading control (lower). Lane M: MW ladder.
  • 14B2C16_PURE_IRF6_Antibody_1_WB_071816
    Total lysates (15 µg protein) from Jurkat (lane 1), HaCaT (lane 2) and NIH3T3 (lane 3) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) purified anti-IRF6 antibody (upper). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306) or HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401) for GAPDH and chemiluminescence detection. GAPDH (poly6314, Cat. No. 631401) antibody was used as a loading control (lower). Lane M: MW ladder.
  • 14B2C16_PURE_IRF6_Antibody_2_ICFC_051316
    HaCat cells were treated with Fixation Buffer and permeabilized with True-Phos™ Perm Buffer, then stained with purified IRF6 (clone 14B2C16) (filled histogram) or purified mouse IgG2a, κ (open histogram), followed by anti-mouse IgG PE.
  • 14B2C16_PURE_IRF6_Antibody_3_IF_010318
    HaCat cells were stained with purified anti-IRF6 clone (14B2C16) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG antibody and Alexa Fluor® 594 conjugated phalloidin (red). Nuclei were stained with DAPI (blue).
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674502 100 µg £182
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Description

IRF6 is a member of the IRF family of transcription factors, which are based on the presence of the conserved IRF tryptophan pentad repeat DNA-binding domain. Unlike other IRF transcription factors, IRF6 is not involved in the expression of interferon genes and doesn’t seem to be involved in innate immunity. Data suggests that IRF6 is necessary for appropriate epidermal differentiation and development. Mutations of IRF6 are associated with human craniofacial developmental defects such as cleft lip, palate, tooth agenesis, Van der Woude syndrome, and popliteal pterygium syndrome. IRF6-deficient mice display severe morphological defects in skin and limbs, and result in perinatal lethality as well.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human IRF6 recombinant protein (92-223 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICFC, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. For intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. For immunocytochemistry, a concentration range of 1.0 - 5.0 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Edwards CL, et al. 2015. Eur J Immunol. 130:45. PubMed
  2. Degen M, et al. 2018. Front Physiol. 9:1703. PubMed
  3. Singh DR, et al. 2022. PLoS Pathog. 18:e1010868. PubMed
RRID
AB_2565216 (BioLegend Cat. No. 674502)

Antigen Details

Structure
467 amino acids with a predicted molecular weight of 53 kD and contains an IRF trytophan pentad repeat that is responsible for DNA binding.
Distribution

Nucleus and cytoplasm.

Function
IRF6 is a transcriptional activator that regulates proliferation and differentiation of both epithelial cells and keratinocytes. IRF6 is required for normal epidermal development during embryogenesis.
Interaction
Interacts with SERPINB5.
Biology Area
Cell Biology, Transcription Factors
Antigen References

1. Melath A, et al. 2013. Asian Pac. J. Cancer Prev. 14:6803.
2. Salahshourifar I, et al. 2012. Eur. J. Med. Genet. 55:389.
3. Blackburn J, et al. 2012. Dev. Biol. 365:61.
4. Botti E, et al. 2011. Proc. Natl. Acad. Sci. USA 108:13710.
5. Restivo G, et al. 2011. EMBO 30:4571.
6. Fleming JA, et al. 2009. Mol. Cell Endocrinol. 299:252.

Gene ID
3664 View all products for this Gene ID
UniProt
View information about IRF6 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All IRF6 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-IRF6 14B2C16 WB,ICFC,ICC
APC anti-IRF6 14B2C16 ICFC
Go To Top Version: 6    Revision Date: 11/28/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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