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Purified anti-IRF7 Antibody

Pricing & Availability
Clone
W16064A (See other available formats)
Regulatory Status
RUO
Other Names
Interferon regulatory factor 7 (IRF-7) protein
Isotype
Rat IgG2a, κ
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Product Citations
publications
W16064A_PURE_IRF7_Antibody_041117
Total cell lysates (15µg protein) from non-treated and recombinant mouse IFN-α treated (100nM, 24h) EL-4 cells were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with 2µg/mL purified anti-IRF7 antibody (clone W16064A) antibody (upper) or loading control GAPDH (clone Poly6314) antibody (lower) . Proteins were visualized by chemiluminescence detection using a goat anti-rat-IgG secondary antibody conjugated to HRP for clone W16064A, and a donkey anti-rabbit IgG Antibody conjugated to HRP for GAPDH. Lane M is the MW ladder.
  • W16064A_PURE_IRF7_Antibody_041117
    Total cell lysates (15µg protein) from non-treated and recombinant mouse IFN-α treated (100nM, 24h) EL-4 cells were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with 2µg/mL purified anti-IRF7 antibody (clone W16064A) antibody (upper) or loading control GAPDH (clone Poly6314) antibody (lower) . Proteins were visualized by chemiluminescence detection using a goat anti-rat-IgG secondary antibody conjugated to HRP for clone W16064A, and a donkey anti-rabbit IgG Antibody conjugated to HRP for GAPDH. Lane M is the MW ladder.
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696402 100 µg £189
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Description

IRF-7 is a member of the interferon regulatory transcription factor family that plays key regulatory roles in innate immunity. Upon virus infection, IRF-7 forms a complex with MyD88 and TRAF6 when stimulated by toll-like receptors TLR7, TLR8, and TLR9, which in turn results in nuclear translocation and induction of interferon gene expression. IRF-7 gene expression is upregulated by the stimulation of interferon through the Jak-STAT signaling pathway, which forms a positive feedback loop. The protein level of IRF-7 after activation is then downregulated by the ubiquitin-proteasome system to avoid excessive production of interferons that may cause damage to the host. Virus induced type I interferon expression was completely abrogated in IRF-7 knockout mice, suggesting that IRF-7 is critical for interferon mediated host defense during virus infection.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial mouse IRF7 recombinant protein (127-487a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 5.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Product Citations
  1. von Gamm M, et al. 2019. J Exp Med. 216:1700. PubMed
RRID
AB_2687056 (BioLegend Cat. No. 696402)

Antigen Details

Structure
457 amino acids, predicted molecular weight of 51.2 kD, contains an IRF tryptophan pentad repeat responsible for DNA binding
Distribution

Shuttles between the cytoplasm and nucleus; activated (phosphorylated) form of IRF-7 accumulates in the nucleus.

Function
Transcription factor that transactivates proinflammatory cytokines and type I interferon genes in response to virus infection.
Interaction
Forms homodimer or heterodimer with IRF-3, interacts with TICAM-1, TICAM-2, MyD88, and TRAF6.
Biology Area
Cell Biology, Transcription Factors
Antigen References

1. Owens BM, et al. 2012. PLoS One. 7:e41050.
2. Yu Y, et al. 2010. Immunity. 33:863.
3. Liang Q, et al. 2011. J. Immunol. 186:1001.
4. Buss C, et al. 2010. J. Immunol. 184:3072.
5. Joo CH, et al. 2007. J. Virol. 81:8282.
6. Balachandran S, et al. 2007. J. Immunol. 178:2429.

Gene ID
54123 View all products for this Gene ID
UniProt
View information about IRF7 on UniProt.org
Go To Top Version: 0    Revision Date: 04/14/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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