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Purified anti-IRF8 Antibody

Pricing & Availability
Clone
W17160D (See other available formats)
Regulatory Status
RUO
Other Names
Interferon Regulatory Factor 8, Interferon Consensus Sequence Binding Protein 1, ICSBP, IRF-8, IMD32A, IMD32B
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W17160D_PURE_IRF8_Antibody_1_072919
Whole cell extracts (15 µg protein) from serum-starved Raw 264.7 cells untreated (-) or treated (+) with 2.5 ng/mL Recombinant Mouse IFN-γ (Cat. No. 575308) for 4 hours were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane and probed with 0.25 µg/mL (1:2000 dilution) of Purified anti-IRF8 Antibody, clone W17160D, overnight at 4°C. Proteins were visualized by chemiluminescence detection HRP anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • W17160D_PURE_IRF8_Antibody_1_072919
    Whole cell extracts (15 µg protein) from serum-starved Raw 264.7 cells untreated (-) or treated (+) with 2.5 ng/mL Recombinant Mouse IFN-γ (Cat. No. 575308) for 4 hours were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane and probed with 0.25 µg/mL (1:2000 dilution) of Purified anti-IRF8 Antibody, clone W17160D, overnight at 4°C. Proteins were visualized by chemiluminescence detection HRP anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • W17160D_PURE_IRF8_Antibody_2_072919
    Whole cell extracts (15 µg protein) from A20 (positive control), EL4, NIH/3T3, RAW264.7 (negative controls), THP-1 and HeLa cells were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane and probed with a 1.0 µg/mL (1:500 dilution) of Purified anti-IRF8 Antibody, clone W17160D, overnight at 4°C. Proteins were visualized by chemiluminescence detection HRP anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • W17160D_PURE_IRF8_Antibody_3_072919
    Whole cell extracts (250 µg total protein) prepared from Raw 264.7 cells treated with 2.5 ng/mL mIFN-γ for 4 hours were immunoprecipitated overnight with 2.0 µg of Purified Rat IgG2b, κ Isotype Control Antibody (Cat. No. 400602) or Purified anti-IRF8 Antibody, clone W17160D. The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a rabbit control antibody against a separate epitope of mouse IRF8. Lane M: Molecular Weight marker.
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623101 25 µg £77
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623102 100 µg £182
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Description

Interferon Regulatory Factor 8 (IRF8) is one of 9 members of the IRF transcription factor family that plays a critical role in the lineage commitment of myeloid cells. IRF8 has a DNA binding domain in its N-terminus region and an IRF association domain (IAD) in the C-terminus region. The IAD allows IRF8 to interact with other transcription factors and regulate gene expression. PU.1 is an IRF8 binding partner that forms a complex with IRF8 and mediates the differentiation of myeloid and lymphoid cells. The IRF8-PU.1 complex binds to a promoter-distal IRF8 binding site and induces the expression of KLF4, which drives the development of Ly6C+ monocytes. IRF8(-/-) mice are immunodeficient and highly  susceptible to infections. They are also characterized by a Chronic Myelogenous Leukemia (CML)-like syndrome marked by an increased number of myeloid progenitor cells that can only commit toward the granulocyte but not toward the macrophage lineage.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant mouse IRF8 protein (amino acids 115-424)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2814480 (BioLegend Cat. No. 623101)
AB_2814480 (BioLegend Cat. No. 623102)

Antigen Details

Structure
Mouse IRF8 is a 424 amino acid protein with a predicted molecular weight of 48.2 kD.
Distribution

Nucleus/Myeolid and lymphoid tissue

Function
Transcription factor
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Innate Immune Signaling
Antigen References
  1. Kurotaki D, et al. 2018. Cell Reports. 22:2628.
  2. Tamura T, et al. 2005. Blood. 106:1938.
  3. Wang H, et al. 2009. Immunol. Res. 43(1-3):109.
Gene ID
3394 View all products for this Gene ID
UniProt
View information about IRF8 on UniProt.org
Go To Top Version: 1    Revision Date: 07/29/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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