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- Clone
- W22128C (See other available formats)
- Regulatory Status
- RUO
- Other Names
- P35568, Insulin receptor substrate 1, IRS-1
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with purified anti-IRS1 (clone W22128C). Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 678351 | 25 µg | £100 | ||||
| 678352 | 100 µg | £281 | ||||
Insulin receptor substrate 1 (IRS1) is a crucial signaling adapter protein that mediates the effects of insulin and insulin-like growth factor 1 (IGF-1) by transmitting signals from their receptors to intracellular pathways such as PI3K/Akt and MAPK. The regulation of IRS1 involves phosphorylation at serine/threonine and tyrosine residues, which modulates its activity and stability. Dysregulation of IRS1 is implicated in several diseases, including type 2 diabetes mellitus and Alzheimer’s disease. In type 2 diabetes, defects in IRS1 signaling contribute to insulin resistance, while in Alzheimer’s disease, altered IRS1 signaling is associated with cognitive decline and neurodegeneration.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant fragment of human IRS1 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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WB - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Antigen Details
- Structure
- Insulin receptor substrate 1 (IRS1) is a 1242 amino acid length protein with a molecular weight of 131.6 kDa. However, due to post-translational modifications like phosphorylation, it often appears as a 180 kDa protein when analyzed via SDS-PAGE.
- Distribution
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Insulin receptor substrate 1 (IRS1) is primarily located in the cytoplasm of the cell. However, upon activation by insulin or IGF-1, it is translocated to the plasma membrane where it interacts with the insulin receptor. It can also be found associated with the plasma membrane and other intracellular membrane-bound organelles as well as the nucleus.
- Function
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- Signal Transduction: Mediates insulin and IGF-1 signaling by transmitting signals from their receptors to intracellular pathways like PI3K/Akt and MAPK.
- Metabolic Regulation: Plays a key role in glucose uptake, lipid metabolism, and overall energy homeostasis
- Cell Growth and Survival: Involved in cellular processes such as growth, differentiation, and survival
- Interaction
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- Insulin Receptor (INSR): IRS1 binds to the insulin receptor via its pleckstrin homology (PH) domain and phosphotyrosine binding (PTB) domain, facilitating downstream signaling.
- IGF-1 Receptor (IGF1R): IRS1 interacts with the IGF-1 receptor, promoting growth and metabolic effects.
- PI3K (Phosphoinositide 3-kinase): IRS1 interacts with the regulatory subunit of PI3K (PIK3R1) through phosphorylated tyrosine residues, activating the PI3K/Akt pathway.
- Grb2 (Growth factor receptor-bound protein 2): This interaction links IRS1 to the MAPK signaling pathway, influencing cell growth and differentiation.
- SOCS7 (Suppressor of cytokine signaling - SOCS7 binds to IRS1 and can inhibit its signaling, acting as a negative regulator.
- JAK2 (Janus kinase 2): IRS1 interacts with JAK2, which is involved in cytokine signaling and can modulate insulin signaling - Antigen References
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- Zheng M, et al. 3Biotech. 11(179): s13205-021-02738-3
- Martinez-Baez A, et al. 2024. Curr Issues Mol Biol. 46:634-649.
- Caruso M, et al. 2014. Diabetes. 63:1933-47.
- Regulation
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- Phosphorylation: Regulated by phosphorylation at serine/threonine and tyrosine residues, which affects its activity and stability
- Feedback Inhibition: Negative feedback mechanisms, often involving serine phosphorylation, can inhibit IRS1 activity to prevent overactivation.
- Protein-Protein Interactions: Interacts with various proteins that modulate its function and localization within the cell - Gene ID
- 3667 View all products for this Gene ID
- UniProt
- View information about IRS1 on UniProt.org
Other Formats
View All IRS1 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-IRS1 | W22128C | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-IRS1
Whole cell extracts (15 µg total protein per lane) from indi...
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