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Purified anti-ITK Antibody

Pricing & Availability
Clone
W15110A (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-2-inducible T-cell kinase, T-cell-specific kinase, EMT, LYK, PSCTK2
Isotype
Mouse IgG1, κ
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Product Citations
publications
W15110A_PURE_ITK_Antibody_1_WB_082018
Total cell lysates (15 µg protein) from Jurkat (lane 1), HeLa (lane 2) and EL4 cells (lane 3) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1 µg/mL (1:500 dilution) of purified anti-ITK antibody, clone W15110A (upper panel). Proteins were visualized by chemiluminescence detection (Cat. No. 426303) using 1:3000 diluted anti-mouse-IgG secondary antibody conjugated to HRP for purified anti-ITK antibody or 1:10000 diluted Direct-Blot™ HRP anti-β-actin antibody, clone W16197A (lower panel). Lane M: Molecular weight ladder.
  • W15110A_PURE_ITK_Antibody_1_WB_082018
    Total cell lysates (15 µg protein) from Jurkat (lane 1), HeLa (lane 2) and EL4 cells (lane 3) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1 µg/mL (1:500 dilution) of purified anti-ITK antibody, clone W15110A (upper panel). Proteins were visualized by chemiluminescence detection (Cat. No. 426303) using 1:3000 diluted anti-mouse-IgG secondary antibody conjugated to HRP for purified anti-ITK antibody or 1:10000 diluted Direct-Blot™ HRP anti-β-actin antibody, clone W16197A (lower panel). Lane M: Molecular weight ladder.
  • W15110A_PURE_ITK_Antibody_2_IF_052516
    Jurkat cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 1% BSA for 60 minutes. Then the cells were intracellularly stained with 5.0 µg/ml purified anti-ITK (clone W15110A) antibody for one hour, followed by staining with Alexa Fluor® 594 conjugated anti-mouse IgG antibody (red) and DAPI (blue) for one hour at room temperature. The image was captured with a 60X objective.
  • W15110A_PURE_ITK_Antibody_ICFC_1_120717
    Human peripheral blood mononuclear cells were surface stained with CD3 Alexa Flour® 647 and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with purified anti-ITK (clone W15110A) (left) or purified mouse IgG1, κ isotype (right). Followed by anti-mouse IgG1 PE.
  • W15110A_PURE_ITK_Antibody_ICFC_2_120717
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687302 100 µg £238
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Description

IL2-inducible T-cell kinase (ITK) is an intracellular tyrosine kinase that is expressed in T cells. It regulates the proliferation, differentiation and development of conventional T cells and NK T cells. This protein has 620 amino acids and contains one PH domain for cell membrane recruitment, one protein kinase domain, and both SH2 and SH3 domains which are commonly found in intracellular kinases. Once T cells are activated, a series of phosphorylation recruits and activates ITK through autophosphorylation. The activated ITK can activate downstream intracellular calcium mobilization and NFAT nucleus translocation, and in turn, activate T cells. The phenotypes of mice deficient in ITK show severely affected immune response and development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human ITK recombinant protein (1-200 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICFC, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For flow cytometric staining, the suggested use of this reagent is ≤ 5.0 µg per million cells in 100 µl volume. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg per ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

RRID
AB_2616942 (BioLegend Cat. No. 687302)

Antigen Details

Structure
620 amino acids with a predicted molecular weight of 71.8 kD. Contains a N-terminal PH domain, a SH2 domain, a SH3 domain, and a C-terminal catalytic kinase domain.
Distribution

Cytoplasm.

Function
ITK is a T-cell specific tyrosine kinase, regulating T cell proliferation and differentiation. It plays an important role in the adaptive immune response.
Interaction
Forms a homo-oligomer. Interacts with PPIA, FASLG, and VAV1.
Biology Area
Cell Biology, Immunology
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Gomez-Rodriguez J, et al. 2016. Nat. Commun. 7:10857.
2. Cho HS, et al. 2015. J. Immunol. 195:4822.
3. Kannan A, et al. 2015. Eur. J. Immunol. 45:2276.
4. Ghosh S, et al. 2014. J. Clin. Immunol. 34:892.
5. Qi Q, et al. 2011. FEBS. J. 278:1970.
6. Prince AL, et al. 2009. Immunol. Rev. 228:115.

Gene ID
3702 View all products for this Gene ID
UniProt
View information about ITK on UniProt.org
Go To Top Version: 2    Revision Date: 08/20/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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