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Purified anti-Jun Phospho (Ser73) Antibody

Pricing & Availability
Clone
A19012A/R/C (See other available formats)
Regulatory Status
RUO
Other Names
Transcription factor Jun, activator protein 1 (AP1), Proto-oncogene c-Jun, transcription factor AP-1 subunit Jun, V-jun avian sarcoma virus 17 oncogene homolog, p39
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A19012A-R-C_PURE_Jun-phospho_Antibody_051222
Whole cell extracts (15 μg protein) from untreated (-) or UV-treated (+) NIH/3T3 cells were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1.0 μg/mL (1:5000 dilution) of purified anti-Jun Phospho (Ser73) for 2 hours at room temperature (left). Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Equal c-Jun loading was confirmed by probing membranes with anti-pan c-Jun (right). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a protein loading control at a 1:10000 dilution. Lane M: Molecular weight marker
  • A19012A-R-C_PURE_Jun-phospho_Antibody_051222
    Whole cell extracts (15 μg protein) from untreated (-) or UV-treated (+) NIH/3T3 cells were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1.0 μg/mL (1:5000 dilution) of purified anti-Jun Phospho (Ser73) for 2 hours at room temperature (left). Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Equal c-Jun loading was confirmed by probing membranes with anti-pan c-Jun (right). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a protein loading control at a 1:10000 dilution. Lane M: Molecular weight marker
  • A19012A-R-C_PURE_Jun-phospho_Antibody_2_051222
    Whole cell extracts (15 μg protein) from UV untreated (-) or treated (+) HeLa cells were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.1 μg/mL (1:5000 dilution) of purified anti-Jun Phospho (Ser73) for 2 hours at room temperature (left). Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a protein loading control at a 1:10000 dilution. Lane M: Molecular weight marker
  • A19012A-R-C_PURE_Jun-phospho_Antibody_3_051222
    NIH/3T3 cells were treated with UV and rested for 35 minutes, fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and treated with lambda protein phosphatase (LPP) (right panel) or left untreated (left panel). Cells were then blocked with 5% FBS for 60 minutes and intracellularly stained with 5.0 µg/mL (1:100 dilution) of purified anti-Jun Phospho (Ser73) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) at 2.5 µg/mL. Nuclei were counterstained with DAPI and the image was captured with a 60X objective. Scale bar: 50 µm
  • A19012A-R-C_PURE_Jun-phospho_Antibody_4_121923
    HeLa cells untreated (negative control, open histogram), or UV-treated (positive control, filled histogram) were fixed and permeabilized using the True-Phos™ Perm Buffer Set (Cat. No. 425401) and intracellularly stained with Purified anti-c-Jun Phospho (Ser73) (clone A19012A/R/C) or Purified Mouse IgG1, κ Isotype Control Antibody (open histogram, dashed line) (representative histogram for untreated or treated cells) (Cat. No. 400101) followed by Alexa Fluor™ 647 Goat anti-mouse IgG (Cat. No. 405322).
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Description

c-Jun is the first oncogenic transcription factor ever discovered. It heterodimerizes with members of the Fos protein family, such as c-Fos, to form the AP-1 transcription factor complex, exhibiting both activating and repressive functions depending on the interacting partner and promoter sequence context. c-Jun is required for cell cycle progression, and is protective against DNA damage, such as that resulting from ultraviolet light exposure. c-Jun is also overexpressed in many cancers including lung, breast and vulvar cancer, and is a target for anti-cancer therapies. Phosphorylation of c-Jun at serine 63 and 73 is activational, and mediated by the MAPK signaling cascade. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide of human c-Jun phosphorylated at Ser73
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 3.0 - 5.0 μg/mL is recommended. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2924613 (BioLegend Cat. No. 605551)
AB_2924613 (BioLegend Cat. No. 605552)

Antigen Details

Structure
c-Jun is a 331 amino acid protein with a predicted molecular weight of 35.7 kD.
Distribution

c-Jun is primarily expressed in the nucleus

Function
Transcription factor
Interaction
c-Jun forms the AP-1 transcription factor complex by heterodimerizing with proteins of the Fos family
Biology Area
Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Transcription Factors
Molecular Family
Phospho-Proteins
Antigen References
  1. Pulverer B, et al. 1991. Nature. 353:670-4. 
  2. Vogt P. 2001.Oncogene. 20:2365-77 .
  3. Brennan A, et al. 2020. J Exp Clin Cancer Res. 39:184. 
Gene ID
3725 View all products for this Gene ID
UniProt
View information about Jun Phospho Ser73 on UniProt.org
Go To Top Version: 2    Revision Date: 12/19/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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