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Purified anti-JunB Antibody

Pricing & Availability
Clone
W20103F (See other available formats)
Regulatory Status
RUO
Other Names
Activator Protein 1, AP-1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W20103F_PURE_JunB_Antibody_1_021122
ICC staining of purified anti-JunB (clone W20103F) on NTERA-2 (negative control, panel A) and HeLa (positive control, panel B) cells. The cells were fixed with 4% PFA and permeabilized with Triton X-100, and blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with 5.0 µg/mL of the primary antibody, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-rat IgG (Cat. No. 405422) for 1 hour at room temperature. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective. Predicted protein expression was obtained from Human Protein Atlas and confirmed with an independent antibody against JunB.
  • W20103F_PURE_JunB_Antibody_1_021122
    ICC staining of purified anti-JunB (clone W20103F) on NTERA-2 (negative control, panel A) and HeLa (positive control, panel B) cells. The cells were fixed with 4% PFA and permeabilized with Triton X-100, and blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with 5.0 µg/mL of the primary antibody, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-rat IgG (Cat. No. 405422) for 1 hour at room temperature. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective. Predicted protein expression was obtained from Human Protein Atlas and confirmed with an independent antibody against JunB.
  • W20103F_PURE_JunB_Antibody_2_021122
    HeLa cells (filled histogram, positive control) and NTERA-2 cells (open histogram, negative control) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401), and intracellularly stained with purified anti-JunB (clone W20103F) followed by PE goat anti-rat IgG (Cat. No. 405406).
  • W20103F_PURE_JunB_Antibody_3_021122
    HeLa cells (filled histogram, positive control) and NTERA-2 cells (open histogram, negative control) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401), and intracellularly stained with purified rat IgG2a, κ isotype control (Cat. No. 400502) followed by PE goat anti-rat IgG (Cat. No. 405406).
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600451 25 µg £81
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600452 100 µg £201
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Description

JunB is a member of the AP-1 family of transcription factors, which also includes c-Jun, c-Fos, Fra1, and JunD. JunB can form dimers with other Jun and Fos proteins, as well as members of the ATF transcription factor family, to form a functional AP-1 transcription factor complex. AP-1 complexes containing JunB can exert both activator and repressor function depending on the interacting partner and promoter sequence context. JunB displays both tumor suppressor and oncogenic activity, inducing genes involved in cell senescence and repressing genes involved in proliferation. JunB is required for T helper type 2 (Th2), T helper 17 (Th17) cell and regulatory T cell (Treg) differentiation. Multiple cancers display aberrant JunB expression and function. In some lymphomas, notably Hodgkin’s lymphoma, JunB expression is elevated and the protein functions as an oncogenic transcription factor. Conversely, loss of JunB is observed in chronic myeloid leukemia.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human JunB protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICC - Quality tested
ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

When using this clone for ICC, we recommend 4% PFA + Triton X-100 for fixation and permeabilization. Other methods tested (4% plus methanol and methanol only) fixation and permeabilization methods produced high non-specific staining.

This clone failed to detect JunB by WB and IHC-P.

RRID
AB_2910470 (BioLegend Cat. No. 600451)
AB_2910470 (BioLegend Cat. No. 600452)

Antigen Details

Structure
JunB is a 347 amino acid protein with a predicted molecular weight of 36 kD.
Distribution

Nucleus

Function
Transcription Factor
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability, Transcription Factors
Antigen References
  1. Shaulian E and Karin M. 2002. Nat Cell Biol. 4:E131-6.
  2. Yang MY, et al. 2003. Blood. 101:3205-11.
  3. Carr T, et al. 2017. Nat Commun. 8:301.
  4. Katagiri T, et al. 2019. Mucosal Immunol. 12:1104-1117.
Gene ID
3726 View all products for this Gene ID
UniProt
View information about JunB on UniProt.org
Go To Top Version: 1    Revision Date: 02/11/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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