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Purified anti-Keratin 15 Antibody

Pricing & Availability
Clone
Poly18339 (See other available formats)
Regulatory Status
RUO
Other Names
Keratin, type I cytoskeletal 15, CK-15, cytokeratin-15, keratin-15, beta, keratin-15, basic keratin-15, K15, cytokeratin 15, cytokeratin15
Isotype
Chicken IgY
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Product Citations
publications
KifLiakath2.png
Whole mount mouse tail epidermis was stained with purified anti-Keratin 14 (green, clone Poly19053), purified anti-Keratin 15 (orange, clone Poly18339) and DAPI (blue). Image generously submitted to the 2017 Cell Life Imaging Competition by Kif Liakath.
  • KifLiakath2.png
    Whole mount mouse tail epidermis was stained with purified anti-Keratin 14 (green, clone Poly19053), purified anti-Keratin 15 (orange, clone Poly18339) and DAPI (blue). Image generously submitted to the 2017 Cell Life Imaging Competition by Kif Liakath.
  • Poly18339_PURE_Keratin15_Antibody_1_IHC_053116
    IHC staining of anti-Keratin 15 antibody (Poly18339) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Sodium Citrate, the tissue was incubated with the primary antibody at 1 µg/ml for 60 minutes at room temperature. After incubation with biotinylated anti-chicken antibody, the tissue was labeled with BioLegend's HRP labeling reagent followed by addition of AEC Chromogen Concentrate. The tissue was then counterstained with hematoxylin, according to the protocol provided.
  • Poly18339_PURE_Keratin15_Antibody_2_WB_053116
    Western blot of anti-Keratin 15 antibody (Poly18339). Lane 1: Molecular weight marker; Lane 2: Human skin lysate. The blot was incubated with the primary antibody at 5 µg/ml for 24 hours at 4°C, followed by incubation with an HRP goat anti-chicken IgY secondary antibody and chemiluminescence detection.
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833904 100 µg £245
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Description

Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 15 is a member of the type I cytokeratin family. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chain. Keratin 15 has been associated with diseases such as malignant syringoma and morpheaform basal cell carcinoma.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Reported Reactivity
Non-Human Primate
Antibody Type
Polyclonal
Host Species
Chicken
Immunogen
This antibody was raised against a peptide sequence derived from the C-terminus of the human keratin 15 protein, CDGQVVSSHKREI.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 μg/ml is suggested. For Western blotting, a concentration range of 1.0 - 10 μg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Moriyama M, et al. 2016. PLoS One. 11:e0164799. PubMed
  2. Ding X, et al. 2016. Nat Commun. 7:13226. PubMed
  3. Moehring F et al. 2018. eLife. 7 pii: e31684. PubMed
  4. Walker H, et al. 2021. Int J Mol Sci. 22:. PubMed
  5. Villarreal-Ponce A, et al. 2020. Cell Rep. 33:108417. PubMed
  6. Li CM, et al. 2020. Cell Reports. 33(13):108566. PubMed
  7. Whisenant D, et al. 2022. Nat Commun. 13:3068. PubMed
RRID
AB_2616894 (BioLegend Cat. No. 833904)

Antigen Details

Structure
Keratin 15 is a 456 amino acid protein with a molecular mass of 49 kD.
Distribution

Tissue distribution: skin.
Cellular distribution: extracellular, nucleus, and cytoskeleton.

Function
Keratin 15 is responsible for maintaining the structural integrity of mammalian epithelial cells.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Antigen References

1. Liu Y, et al. 2003. J. Invest. Dermatol. 121(5)963. PubMed
2. Jih DM, et al. 1999. J. Cutan. Pathol. 26(3):113. PubMed
3. Leube RE, et al. 1988. J. Cell Biol. 106(4):1249. PubMed

Gene ID
3866 View all products for this Gene ID
UniProt
View information about Keratin 15 on UniProt.org
Go To Top Version: 3    Revision Date: 08/12/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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