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Purified anti-LAT Antibody

Pricing & Availability
Clone
11B.12 (See other available formats)
Regulatory Status
RUO
Other Names
Linker for activation of T-cells family member 1, pp36, p36-38
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_11Bdot12_Purified_LAT_Antibody_WB_2_123118.png
Total cell lysates (15 µg total protein) from HeLa (negative control), Jurkat, and EL4 cells were resolved by 4-20% Tris-Gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-LAT Antibody, clone 11B.12, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 664804) was used as a loading control at a 1:5000 dilution (lower).
  • 1_11Bdot12_Purified_LAT_Antibody_WB_2_123118.png
    Total cell lysates (15 µg total protein) from HeLa (negative control), Jurkat, and EL4 cells were resolved by 4-20% Tris-Gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-LAT Antibody, clone 11B.12, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 664804) was used as a loading control at a 1:5000 dilution (lower).
  • 2_11B12_WB_092608
    Western blot analysis of extracts from Jurkat cells (lane 1) and mouse thymocytes (lane 2) using anti-LAT, clone 11B.12.
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641102 100 µg £157
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Description

LAT is a transmembrane molecule that mediates signaling following the engagement of a number of cell surface receptors including TCR during mature T cell activation, pre-TCR during T cell development, Fc RIII on NK cells, and FcεRI on mast cells.LAT is multiply phosphorylated on tyrosines by ZAP-70 and Syk, which leads to downstream effects that include release of intracellular calcium, PKC activation, MAPK activation and cytoskeletal reorganization.Palmitoylation of LAT is required for its localization to lipid rafts and efficient phosphorylation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
GST fusion protein corresponding to residues 31-233 of human LAT, cytoplasmic domain.
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store between 2°C and 8°C
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone may recognize a ~80 kD protein of unknown origin in some cell lines and tissue homogenates. It is also predicted to recognize rat LAT due to complete sequence homology between human, mouse, and rat LAT.

Application References

(PubMed link indicates BioLegend citation)
  1. Dorn T, et al. 2007. Blood 109:2346.
  2. Sloan D, et al. 2006. J. Immunol. 176:1825
Product Citations
  1. Deswal S, et al. 2011. PLoS One. 6:e22928. PubMed
  2. Chen EW, et al. 2019. Front Immunol. 10:1718. PubMed
RRID
AB_2157732 (BioLegend Cat. No. 641102)

Antigen Details

Structure
Type III transmembrane protein, MW of 28 kD but expected at 36-38 kD due to post-translational modifications including palmitoylation; multiple isoforms with a predicted MW of 25-28 kD reported.
Distribution

Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).

Function
LAT is required for signaling through a number of cell surface receptors including TCR, FcγRIII, and FcεRI.
Interaction
Interacts directly with GRB2, GRAP, GRAP2, PLCG1 and PLCG2. Interacts indirectly with CBL, SOS, VAV, LCP2, SHB, SKAP2, MIST,and FcγR1A
Cell Type
Mast cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Phospho-Proteins
Antigen References

1. Zhang W et al. 1998. Cell 92:83.
2. Weber JR. et al. 1998. J. Exp. Med. 187:1157

Regulation
Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation
Gene ID
27040 View all products for this Gene ID
UniProt
View information about LAT on UniProt.org
Go To Top Version: 3    Revision Date: 12/14/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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