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Purified anti-LDHB Antibody

Pricing & Availability
Clone
Poly28603 (See other available formats)
Regulatory Status
RUO
Other Names
LDHB
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly28603_Purified_LDHB_Antibody_1_040919.png
Western blot of purified anti-LDHB antibody (Poly28603). Lane 1: Molecular weight marker; Lane 2: 20µg of human brain lysate; Lane 3: 20µg of mouse brain lysate; Lane 4: 20µg of rat brain lysate. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled Donkey anti-Rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28603_Purified_LDHB_Antibody_1_040919.png
    Western blot of purified anti-LDHB antibody (Poly28603). Lane 1: Molecular weight marker; Lane 2: 20µg of human brain lysate; Lane 3: 20µg of mouse brain lysate; Lane 4: 20µg of rat brain lysate. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled Donkey anti-Rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28603_Purified_LDHB_Antibody_2_040919.png
    IHC staining of purified anti-LDHB antibody (Poly28603) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • Poly28603_Purified_LDHB_Antibody_3_040919.png
    IHC staining of purified anti-LDHB antibody (Poly28603) on formalin-fixed paraffin-embedded mouse kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • Poly28603_Purified_LDHB_Antibody_4_040919.png
    IHC staining of purified anti-LDHB antibody (Poly28603) on formalin-fixed paraffin-embedded rat kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
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860301 25 µg £86
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Description

Lactate Dehydrogenase B (LDHB) is one of the subunits of the lactate dehydrogenase enzyme that catalyzes the synthesis of (S)-Lactate from pyruvate. LDHB was shown to be a downstream mediator of the mTOR signaling pathway that plays a critical role in hyperactive mTOR-mediated tumorigenesis, indicating it as potential therapeutic target for cancer and other diseases caused by aberrant mTOR signaling.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Recombinant Lactate Dehydrogenase B (LDHB) expressed in E.coli.
Formulation
0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. For formalin-fixed paraffin-embedded immunohistochemical staining, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2801230 (BioLegend Cat. No. 860301)

Antigen Details

Structure
LDHB is a 334 amino acid protein with an apparent molecular mass of ~37 kD.
Distribution

Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Cytosol

Function
LDHB is part of the lactate synthesis pathway that synthesizes S-Lactate from pyruvate
Interaction
Forms Homotetramers
Cell Type
Astrocytes, Neurons, Oligodendrocytes
Biology Area
Cancer Biomarkers, Neurodegeneration, Neuroscience, Synaptic Biology
Molecular Family
Tumor Suppressors
Antigen References
  1. Swiderek K and Paneth P. 2011. Arch. Biochem Biophys. 505(1):33
  2. Zha X, et al. 2011. Cancer Res. 71(1):13
Gene ID
3945 View all products for this Gene ID 16832 View all products for this Gene ID 24534 View all products for this Gene ID
UniProt
View information about LDHB on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 04/26/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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