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Purified anti-LDHB Antibody

Pricing & Availability
Clone
BL28598 (See other available formats)
Regulatory Status
RUO
Other Names
LDHB
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
BL28598_Purified_LDHB_Antibody_1_042919.png
Western blot of purified anti-LDHB antibody (BL28598). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • BL28598_Purified_LDHB_Antibody_1_042919.png
    Western blot of purified anti-LDHB antibody (BL28598). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • BL28598_Purified_LDHB_Antibody_2_042919.png
    IHC staining of purified anti-LDHB antibody (clone BL28598) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • BL28598_Purified_LDHB_Antibody_3_042919.png
    IHC staining of purified anti-LDHB antibody (clone BL28598) on formalin-fixed paraffin-embedded mouse kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • BL28598_Purified_LDHB_Antibody_4_042919.png
    IHC staining of purified anti-LDHB antibody (clone BL28598) on formalin-fixed paraffin-embedded rat kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
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859801 25 µg £73
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Description

Lactate Dehydrogenase B (LDHB) is one of the subunits of the lactate dehydrogenase enzyme that catalyzes the synthesis of (S)-Lactate from pyruvate.  LDHB was shown to be a downstream mediator of the mTOR signaling pathway that plays a critical role in hyperactive mTOR-mediated tumorigenesis, indicating it as potential therapeutic target for cancer and other diseases caused by aberrant mTOR signaling.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Lactate Dehydrogenase B (LDHB) expressed in E.coli
Formulation
0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application. For formalin-fixed paraffin-embedded immunohistochemical staining, a concentration range of 1 - 5 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2810734 (BioLegend Cat. No. 859801)

Antigen Details

Structure
LDHB is a 334 amino acid protein with an apparent molecular mass of ~37 kD.
Distribution

Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Cytosol

Function
LDHB is part of the lactate synthesis pathway that synthesizes S-Lactate from pyruvate
Interaction
Forms Homotetramers
Cell Type
Mature Neurons
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers
Molecular Family
Enzymes and Regulators
Antigen References
  1. Swiderek K and Paneth P. 2011. Arch. Biochem Biophys. 505(1):33
  2. Zha X, et al. 2011. Cancer Res. 71(1):13
Gene ID
3945 View all products for this Gene ID 16832 View all products for this Gene ID 24534 View all products for this Gene ID
UniProt
View information about LDHB on UniProt.org
Go To Top Version: 2    Revision Date: 08/29/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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