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- Clone
- M1905E01 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Obese Protein, Obesity Factor, LEPD, OB, OBS
- Isotype
- Mouse IgG2a, λ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Western blot analysis of recombinant human leptin 25 ng (lane 1), and 50 ng (lane 2) using anti-Leptin antibody (clone M1905E01) and HRP anti-mouse IgG antibody.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 675002 | 100 µg | £182 | ||||
Leptin is primarily synthesized from adipocytes and plays a major role in energy homeostasis and obesity. Leptin resistance has been proposed as a pathophysiological mechanism of obesity. In obese individuals, expression of the short form of the leptin receptor, Ob-Ra, is downregulated, and the individual may be unresponsive to leptin signals. Leptin has also been implicated in the regulation of reproduction, glucose homeostasis, bone formation, hematopoiesis, and wound healing.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human Leptin.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
-
- RRID
-
AB_2565487 (BioLegend Cat. No. 675002)
Antigen Details
- Structure
- 167 amino acids with a predicted molecular weight of approximately 18 kD.
- Distribution
-
Secreted by adipocytes, gastric epithelial cells, or fibroblasts.
- Function
- Plays a major role in energy homeostasis and obesity.
- Interaction
- Interacts with Siglec-6.
- Ligand/Receptor
- Leptin receptors.
- Cell Type
- Epithelial cells, Fibroblasts
- Biology Area
- Angiogenesis, Cell Biology, Signal Transduction
- Molecular Family
- Enzymes and Regulators
- Antigen References
-
1. Ando S, et al. 2014. Front Oncol. 4:340.
2. Upadhyay J, et al. 2015. Metabolism 64:105.
3. Sainz N, et al. 2015. Metabolism 64:35.
4. Zhang F, et al. 1997. Nature 387:206.
5. Brennan AM, et al. 2006. Nat. Clin. Pract. Endocrinol. Metab. 2:318.
6. Halaas JL, et al. 1995. Science 269:543. - Gene ID
- 3952 View all products for this Gene ID
- UniProt
- View information about Leptin on UniProt.org
Other Formats
View All Leptin Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Leptin | M1905E01 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Leptin
Western blot analysis of recombinant human leptin 25 ng (lan...
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