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Purified anti-Lin28A Antibody

Pricing & Availability
Clone
14E6-4E6 (See other available formats)
Regulatory Status
RUO
Other Names
Protein Lin-28 Homolog A,Lin-28A, CSDD1,ZCCHC1, Zinc Finger CCHC Domain-Containing Protein 1
Isotype
Mouse IgG2a, κ
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Product Citations
publications
14E6-4E6_Purified_Lin28A_Antibody_WB_101716
Total cell lysate from HeLa cells (lane 1, 15 µg), NTERA2 (lane 2, 15 µg) and NF1 (lane 3, 15 µg) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with purified anti-Lin28A antibody (clone 14E6-4E6). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
  • 14E6-4E6_Purified_Lin28A_Antibody_WB_101716
    Total cell lysate from HeLa cells (lane 1, 15 µg), NTERA2 (lane 2, 15 µg) and NF1 (lane 3, 15 µg) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with purified anti-Lin28A antibody (clone 14E6-4E6). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
  • 2_14E6-4E6_Purified_Lin28A_Antibody_WB_2_070218
    Whole cell lysates (15 µg protein) from HeLa (negative control), HepG2 (negative control), NTERA-2, and HEL (negative control) and cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to a nitrocellulose membrane, and probed with 0.1 µg/mL (1:5000 dilution) of purified anti-Lin28A antibody, clone 14E6-4E6. Proteins were visualized using chemiluminescence detection by incubating the membrane with a 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH antibody (1:2000 dilution, Cat. No. 649203) was used as a loading control (lower). Lane M: Molecular Weight Marker.
  • 3_14E6-4E6_Purified_Lin28A_Antibody_IF_011515
    NTERA‐2 cells were fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X‐100 for 5 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2.5 µg/mL Lin28A antibody (14E6‐4E6) and followed by DyLight™ 594 goat anti‐mouse IgG for one hour at room temperature. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
  • 4_14E6-4E6_Purified_Lin28A_Antibody_ICFC_032322
    HeLa cells (negative target) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401), and intracellularly stained with 0.25 µg of purified anti-LIN28A antibody (clone 14E6-4E6) (filled histogram) or 0.25 µg of Purified Mouse IgG1, κ Isotype Ctrl Antibody (Cat. No. 400102) (open histogram) followed by PE goat anti-mouse IgG antibody (Cat. No. 405307).
  • 5_14E6-4E6_Purified_Lin28A_Antibody_ICFC_032322
    NTERA2 cells (positive target) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401), and intracellularly stained with 0.25 µg of purified anti-LIN28A antibody (clone 14E6-4E6) (filled histogram) or 0.25 µg of Purified Mouse IgG1, κ Isotype Ctrl Antibody (Cat. No. 400102) (open histogram) followed by PE goat anti-mouse IgG antibody (Cat. No. 405307)
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657802 100 µg £238
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Description

LIN28A is a highly conserved RNA-binding protein and reprogramming factor that plays important roles in development, self-renewal of stem cells, glucose metabolism and tumorigenesis. Lin28A modulates not only miRNAs, particularly the let-7 family miRNAs, but also the translation of mRNAs that are important for cell growth and metabolism. LIN28A/B has been observed to be overexpressed in a wide range of solid tumors and hematological malignancies, and has emerged as a new therapeutic target candidate for cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Lin28A from E. coli
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.025 - 1.0 µg per ml (1:500 - 1:20,000 dilution). For immunocytochemistry, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For flow cytometric staining, the suggested use of this reagent is 0.06 - 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

HepG2 and HEL cells were selected for specificity testing because they express LIN28B mRNA but lack detectable LIN28A expression (source: Human Protein Atlas).

This clone is specific for Lin28A does not recognize Lin28B.

RRID
AB_2562650 (BioLegend Cat. No. 657802)

Antigen Details

Structure
209 amino acids, predicted molecular weight of 28 kD with the characteristic of a cold shock domain (CSD) and two tandem Cys-Cys-His-Cys (CCHC)-type zinc-binding motifs (CCHCx2)
Distribution

Cytoplasm and nucleus; localizes to cytoplasmic processing bodies and stress granules

Function
Development, self-renewal of stem cells, glucose metabolism and tumorigenesis; regulates Let-7 family miRNA, translation of mRNA
Interaction
EIF3S2, NCL and ZCCHC11/TUT4
Cell Type
Embryonic Stem Cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics, Immunology, Protein Synthesis, Signal Transduction, Stem Cells
Antigen References

1. Shyh-Chang N, et al. 2013. Cell Stem Cell. 12:395.
2. Thornton JE, et al. 2012. Trends Cell Biol. 22:474.
3. Huang Y. 2012. Wiley Interdiscip. Rev. RNA. 3:483.
4. Cho J, et al. 2012. Cell. 151:765.
5. Li N, et al. 2012. J. Biol. Chem. 287:17386.
6. Zhu H, et al. 2011. Cell 147:81.

Gene ID
79727 View all products for this Gene ID
UniProt
View information about Lin28A on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 03/23/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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