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Purified anti-LRP2 Antibody (Previously Covance catalog# MMS-5120)

Pricing & Availability
Clone
CD7D5 (See other available formats)
Regulatory Status
RUO
Other Names
Low-density lipoprotein receptor-related protein 2, LRP-2, megalin, glycoprotein 330, Heymann nephritis antigen homolog, calcium sensor protein
Previously
Covance Catalog# MMS-5120
Isotype
Mouse IgG1
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Product Citations
publications
CD7D5_PURE_LRP2_MuKidney_IHCP_052515
IHC staining of purified anti-LRP2 antibody (clone CD7D5) on formalin-fixed paraffin-embedded mouse kidney tissue. Following antigen retrieval using Protease 8, the tissue was incubated with 10 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • CD7D5_PURE_LRP2_MuKidney_IHCP_052515
    IHC staining of purified anti-LRP2 antibody (clone CD7D5) on formalin-fixed paraffin-embedded mouse kidney tissue. Following antigen retrieval using Protease 8, the tissue was incubated with 10 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • CD7D5_PURE_LRP2_RatKidney_IHCP_052515
    IHC staining of purified anti-LRP2 antibody (clone CD7D5) on formalin-fixed paraffin-embedded rat kidney tissue. Following antigen retrieval using Protease 8, the tissue was incubated with 10 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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826901 100 µL £189
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Description

Megalin or low density lipoprotein-related protein 2 (LRP2), is a multi-ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithelial tissues such as the kidney. LRP2 mediates uptake of Apo-J which is a binding protein for Amyloid beta. Megalin has a large amino-terminal extracellular domain, a single transmembrane domain, and a short carboxy-terminal cytoplasmic tail. The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase. Megalin is critical for the reuptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. It also has a role in cell-signaling; extracellular ligands include parathyroid hormones and the morphogen sonic hedgehog while cytosolic ligands include MAP kinase scaffold proteins and JNK interacting proteins. Recycling of this membrane receptor is regulated by phosphorylation of its cytoplasmic domain. Mutations in the LRP2 gene cause Donnai-Barrow syndrome (DBS) and facio-oculoacoustico-renal syndrome (FOAR).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against purified human LRP2 protein.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

IHC-P - Quality tested
IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunohistochemistry (IHC-P and IHC-F).

Application References

(PubMed link indicates BioLegend citation)
  1. Nashida T, et al. 2013. Arch. Biochem. Biophys. 530(1)32-9. (IHC-F)
RRID
AB_2564906 (BioLegend Cat. No. 826901)

Antigen Details

Biology Area
Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
Apolipoproteins
Gene ID
14725 View all products for this Gene ID 29216 View all products for this Gene ID
UniProt
View information about LRP2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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