Western blot of purified anti-MAG antibody (clone B11F7). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate; Lane 5: 20 µg of Hela cell lysate. The blot was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C, followed by the incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). HRP anti-β-actin antibody (clone 2F1-1, Cat. No. 643807) was used as a loading control. Enhanced chemiluminescence was used as the detection system.
Western blot of purified anti-MAG antibody (clone B11F7). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate; Lane 5: 20 µg of Hela cell lysate. The blot was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C, followed by the incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). HRP anti-β-actin antibody (clone 2F1-1, Cat. No. 643807) was used as a loading control. Enhanced chemiluminescence was used as the detection system.
Western blot of purified anti-MAG antibody (clone B11F7). Lane 1: Molecular weight marker; Lane 2: 20 µg of HEK293T cell lysate overexpressing human MAG; Lane 3: 20 µg of control HEK293T cell lysate. The blot was incubated with 1 µg/ml of the primary antibody overnight at 4°C, followed by the incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
IHC staining of purified anti-MAG antibody (clone B11F7) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
IHC staining of purified anti-MAG antibody (clone B11F7) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
IHC staining of purified anti-MAG antibody (clone B11F7) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Myelin-associated glycoprotein (MAG) is a cell membrane glycoprotein and a member of the siglec (sialic acid-binding immunoglobulin-like lectins) family. MAG has two isoforms in myelinated structures in the nervous system. MAG is expressed on oligodendrocytes and Schwann cells and is believed to be involved in myelination during nerve regeneration. MAG knockout mice develop quite severe pathological abnormalities associated with myelin instability.
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application References
(PubMed link indicates BioLegend citation)
Dobersen MJ, et al. 1985. Neurochem Research. 4: 499 (WB and IHC)
MAG is a 626 amino acid protein with a molecular mass of 70 kD.
Distribution
Tissue distribution: Central and peripheral nervous system, in periaxonal myelin and at Schmidt-Lanterman incisures.
Cellular distribution: Plasma membrane
Function
MAG is involved in the process of axon myelination and maintenance of myelin sheath along axons. MAG also functions as a receptor that promotes differentiation, maintenance and survival of oligodendrocytes. Aberrant immunoreactivity to MAG has been suggested to play a role in the pathogenesis of multiple sclerosis.
Interaction
MAG binds to neuronal sialic acid-containing gangliosides and to glycoproteins RTN4R and RTN4RL2.
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