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Purified anti-MALT1 Antibody

Pricing & Availability
Clone
W20127A (See other available formats)
Regulatory Status
RUO
Other Names
MALT1 Paracaspase, PCASP1, MLT, Mucosa-Associated Lymphoid Tissue Lymphoma Translocation Protein 1, Mucosa Associated Lymphoid Tissue Lymphoma Translocation Gene 1, MALT1 Protease, MALT Lymphoma-Associated Translocation, Caspase-Like Protein, MLT1
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W20127A_PURE_MALT1_Antibody_1_112922
IHC staining using purified anti-MALT1 (clone W20127A) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using 1X Tris-Buffered Saline with Tween 20 (Cat. No. 925501), the tissue was incubated with (panel B) or without (panel A) 10 μg/mL anti-MALT1 (clone W20127A) overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-rat IgG (Cat. No. 405416) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar = 50 µm
  • W20127A_PURE_MALT1_Antibody_1_112922
    IHC staining using purified anti-MALT1 (clone W20127A) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using 1X Tris-Buffered Saline with Tween 20 (Cat. No. 925501), the tissue was incubated with (panel B) or without (panel A) 10 μg/mL anti-MALT1 (clone W20127A) overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-rat IgG (Cat. No. 405416) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar = 50 µm
  • W20127A_PURE_MALT1_Antibody_1_112922
    ICC staining of purified anti-MALT1 (clone W20127A) on Ramos cells. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.5% Triton X, and blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with 5.0 ug/mL biotin rat IgG2a, κ isotype control (Cat. No. 400503) (panel A) or 5.0 µg/mL anti-MALT1 (clone W20127A) (panel B) overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-rat IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the image was captured with a 60X objective. Scale bar = 50 µm
Cat # Size Price Quantity Check Availability Save
607251 25 µg £92
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607252 100 µg £238
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Description

MALT1 is a ubiquitously expressed protease that is localized in the cytoplasm as a catalytically inactive BCL10-bound proenzyme. MALT1 acts downstream of oligomeric CARMA/CARD-BCL10-MALT1 (CBM) complexes to regulate various functions in which MALT1 serves a dual purpose. Firstly, MALT1 is a scaffolding protein that plays an integral role in the regulation of the NF-κB and MAP kinase p38 pathways. In addition to serving as a scaffolding protein, MALT1 also possesses protease activity which promotes lymphocyte activation by cleavage of signaling proteins. Furthermore, MALT1 possesses a caspase-like domain with two conserved residues, cysteine 464 and histidine 415, which act as catalytic mechanisms in caspases and cysteine proteases ultimately contributing to inflammation, cell death, and cell proliferation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human MALT-1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For ICC, we recommend fixation with 4%PFA, followed by permeabilization with 0.5% Triton-X or 100% ice-cold methanol. We do not recommend methanol fixation. 

This antibody is not suitable for WB due to poor target selectivity.

RRID
AB_2927965 (BioLegend Cat. No. 607251)
AB_2927965 (BioLegend Cat. No. 607252)

Antigen Details

Structure
MALT1 is an 824 amino-acid protein with a predicted molecular weight of 92 kD.
Distribution

MALT1 is ubiquitously expressed in the cytoplasm. 

Function
Scaffolding protein, protease
Interaction
MALT1 acts downstream of CARMA/CARD-BCL10-MALT1 (CBM) complexes to regulate NF-kappa-B and MAP kinase p38 pathways.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Proliferation and Viability
Molecular Family
Enzymes and Regulators, Proteases, Scaffold Proteins
Antigen References
  1. Beyaert R, et al. 2015. FEBS J. 282:3286-97.
  2. Thome M, et al. 2018. Front Immunol. 9:1927.
  3. Hailfinger S, et al. 2016. J Invest Dermatol. 136:788-79.
Gene ID
10892 View all products for this Gene ID
UniProt
View information about MALT1 on UniProt.org
Go To Top Version: 1    Revision Date: 10/21/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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